CD133 expression and identification of CD133/nestin positive cells in rhabdomyosarcomas and rhabdomyosarcoma cell lines
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22156015
PubMed Central
PMC4605712
DOI
10.3233/acp-2011-0018
PII: 9251101917777980
Knihovny.cz E-zdroje
- MeSH
- antigen AC133 MeSH
- CD antigeny metabolismus MeSH
- dítě MeSH
- fluorescenční protilátková technika MeSH
- glykoproteiny metabolismus MeSH
- kmenové buňky metabolismus MeSH
- kohortové studie MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nádorové biomarkery metabolismus MeSH
- nádorové buněčné linie MeSH
- nestin MeSH
- peptidy metabolismus MeSH
- předškolní dítě MeSH
- proteiny intermediálních filament metabolismus MeSH
- proteiny nervové tkáně metabolismus MeSH
- regulace genové exprese u nádorů MeSH
- rhabdomyosarkom genetika metabolismus patologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigen AC133 MeSH
- CD antigeny MeSH
- glykoproteiny MeSH
- nádorové biomarkery MeSH
- NES protein, human MeSH Prohlížeč
- Nes protein, mouse MeSH Prohlížeč
- nestin MeSH
- peptidy MeSH
- PROM1 protein, human MeSH Prohlížeč
- Prom1 protein, mouse MeSH Prohlížeč
- proteiny intermediálních filament MeSH
- proteiny nervové tkáně MeSH
BACKGROUND: Co-expression of CD133, cell surface glycoprotein, and nestin, an intermediate filament protein, was determined to be a marker of neural stem cells and of cancer stem cells in neurogenic tumors. METHODS: We examined the expression of CD133 and nestin in ten tumor tissue samples taken from patients with rhabdomyosarcomas and in five rhabdomyosarcoma cell lines. Immunohistochemistry and immunofluorescence were used to examine FFPE tumor tissue samples. Cell lines were analyzed by immunofluorescence, immunoblotting, flow cytometry, and RT-PCR. Functional assays (clonogenic in vitro assay and tumorigenic in vivo assay) were also performed using these cell lines. RESULTS: CD133 and nestin were detected in all 10 tumor tissue samples and in all 5 cell lines; however, the frequency of CD133+, Nes+, and CD133+/Nes+ cells, as well as the intensity of fluorescence varied in individual samples or cell lines. The expression of CD133 and nestin was subsequently confirmed in all cell lines by immunoblotting. Furthermore, we observed an increasing expression of CD133 in relation to the cultivation. All cell lines were positive for Oct3/4 and nucleostemin; NSTS-11 cells were also able to form xenograft tumors in mice. CONCLUSION: Our results represent the first evidence of CD133 expression in rhabdomyosarcoma tissue and in rhabdomyosarcoma cell lines. In addition, the co-expression of CD133 and nestin as well as results of the functional assays suggest a possible presence of cancer cells with a stem-like phenotype in these tumors.
Citace poskytuje Crossref.org
Atypical nuclear localization of CD133 plasma membrane glycoprotein in rhabdomyosarcoma cell lines
Nestin as a marker of cancer stem cells
