Analysis of D1853N ATM polymorphism in radiosensitive patients with cervical carcinoma
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Ataxia Telangiectasia Mutated Proteins MeSH
- DNA-Binding Proteins genetics MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Mutagenesis, Site-Directed MeSH
- Tumor Suppressor Proteins genetics MeSH
- Uterine Cervical Neoplasms genetics radiotherapy MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Polymorphism, Genetic * MeSH
- Protein Serine-Threonine Kinases genetics MeSH
- Cell Cycle Proteins genetics MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology, Nucleic Acid MeSH
- Carcinoma, Squamous Cell genetics radiotherapy MeSH
- Radiation Tolerance genetics MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ATM protein, human MeSH Browser
- Ataxia Telangiectasia Mutated Proteins MeSH
- DNA-Binding Proteins MeSH
- Tumor Suppressor Proteins MeSH
- Protein Serine-Threonine Kinases MeSH
- Cell Cycle Proteins MeSH
UNLABELLED: Clinical oncologists have been focusing their efforts on attempting to define risk groups of patients with unusual biological reactions to the recommended therapy regimens using molecular biology techniques. THE AIMS OF OUR STUDY WERE: (i) to find a design and validate a method for fast and reliable analysis of the D1853N (5557G>A) genetic polymorphism in the ATM (ataxia-telangiectasia mutated) gene; (ii) to use side-directed mutagenesis to generate ATM 5557A-positive DNA (reference ATM5557A DNA); and (iii) to analyze a group of patients suffering from cervical carcinoma with adverse responses to radiotherapy. The 5557A variant was found in three of twenty women (15%). Our data show that the prevalence of the 5557A allelic variant in cervical cancer subjects with adverse responses after irradiation probably does not differ from the prevalence common in Caucasians. A larger population study should confirm these preliminary results.
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