Correcting the axial shrinkage of skeletal muscle thick sections visualized by confocal microscopy
Language English Country Great Britain, England Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Capillaries anatomy & histology ultrastructure MeSH
- Microscopy, Confocal methods MeSH
- Muscle Fibers, Skeletal ultrastructure MeSH
- Muscle, Skeletal blood supply cytology MeSH
- Rats MeSH
- Image Processing, Computer-Assisted MeSH
- Rats, Wistar MeSH
- Imaging, Three-Dimensional methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
Confocal microscopy is a suitable method for measurements and visualization of skeletal muscle fibres and the neighbouring capillaries. When using 3D images of thick sections the tissue deformation effects should be avoided. We studied the deformation in thick sections of the rat skeletal muscle from complete stacks of images captured with confocal microscope. We measured the apparent thickness of the stacks and compared it to the slice thickness deduced from calibrated microtome settings. The ratio of both values yielded the axial scaling factor for every image stack. Careful sample preparation and treatment of the tissue cryosections with cold Ringer solution minimize the tissue deformation. We conclude that rescaling by the inverse of the axial scaling factor of the stack of optical slices in the direction of the microscope optical axis satisfactorily corrects the axial deformation of skeletal muscle samples.
References provided by Crossref.org
Fiber-type composition and 3D capillary analysis of the human splenius capitis muscle
3D analysis of capillary network in skeletal muscle of obese insulin-resistant mice
Capillary Network Morphometry of Pig Soleus Muscle Significantly Changes in 24 Hours After Death
