Group B Streptococcus (GBS) causes severe infections in infants and in immunocompromised adults. GBS pathogenicity varies between and within serotypes, with considerable variation in genetic content between strains. For this reason, it is important to be able to carry out immediate and comprehensive diagnostics of these infections. Seven genes important for screening of GBS infection were detected: cfb gene encoding the CAMP factor presented in every GBS; the cps operon genes such as cps1aH, cps1a/2/3IJ, and cps5O specific for capsular polysaccharide types Ia, III, and V, respectively; macrolide resistance genes ermB and mefA/E; and the gbs2018 S10 region specific for ST17 hypervirulent clone. Standardization of multiplex PCR with the use of seven primer pairs was performed on 81 bacterial strains representing different GBS isolates (n = 75) and other Gram-positive cocci (n = 10). Multiplex PCR can be used as an effective screening method to detect different sequences important for the screening of GBS infection.

Jagiellonian University Medical College Cracow Poland

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Brimil N, Barthell E, Heindrichs U, Kuhn M, Lütticken R, Spellerberg B. Epidemiology of Streptococcus agalactiae colonization in Germany. Int J Med Microbiol. 2006;296:39–44. doi: 10.1016/j.ijmm.2005.11.001. PubMed DOI

Brzychczy-Włoch M, Gosiewski T, Bodaszewska M, Pabian W, Bulanda M, Kochan P, Strus M, Heczko PB. Genetic characterization and diversity of Streptococcus agalactiae isolates with macrolide resistance. J Med Microbiol. 2010;59:780–786. doi: 10.1099/jmm.0.018176-0. PubMed DOI

Brzychczy-Włoch M, Gosiewski T, Bodaszewska-Lubas M, Adamski P, Heczko PB. Molecular characterization of capsular polysaccharides and surface protein genes in relation to genetic similarity of group B streptococci isolated from Polish pregnant women. Epidemiol Infect. 2012;140:329–336. doi: 10.1017/S0950268811000616. PubMed DOI

Centers for Disease Control and Prevention (CDC) Trends in perinatal group B streptococcal disease—United States, 2000–2006. MMWR Morb Mortal Wkly Rep. 2009;58:109–112. PubMed

Cogswell FB, Bantar CE, Hughes TG, Gu Y, Philipp MT. Host DNA can interfere with detection of Borrelia burgdorferi in skin biopsy specimens by PCR. J Clin Microbiol. 1996;34:980–982. PubMed PMC

Domeika M, Drulyte O. Use of PCR for the detection of genital Chlamydia trachomatis infection on self-obtained mailed vaginal samples. Acta Obstet Gynecol Scand. 2000;79:570–575. doi: 10.1080/j.1600-0412.2000.079007570.x. PubMed DOI

Gherardi G, Imperi M, Baldassarri L, Pataracchia M, Alfarone G, Recchina S, Orefici G, Dicuonzo G, Creti R. Molecular epidemiology and distribution of serotypes, surface proteins, and antibiotic resistance among group B streptococci in Italy. J Clin Microbiol. 2007;45:2909–2916. doi: 10.1128/JCM.00999-07. PubMed DOI PMC

Johri AK, Paoletti LC, Glaser P, Dua M, Sharma PK, Grandi G, Rappuoli R. Group B Streptococcus: global incidence and vaccine development. Nat Rev Microbiol. 2006;4:932–942. doi: 10.1038/nrmicro1552. PubMed DOI PMC

Jones N, Bohnsack JF, Takahashi S, Oliver KA, Chan MS, Kunst F, Glaser P, Rusniok C, Crook DW, Harding RM, Bisharat N, Spratt BG. Multilocus sequence typing system for group B Streptococcus. J Clin Microbiol. 2003;41:2530–2536. doi: 10.1128/JCM.41.6.2530-2536.2003. PubMed DOI PMC

Ke D, Menard C, Picard FJ, Boissinot M, Ouellette M, Roy PH, Bergeron MG. Development of conventional and real-time PCR assays for the rapid detection of group B streptococci. Clin Chem. 2000;46:324–331. PubMed

Kotarski J, Heczko PB, Lauterbach R, Niemiec T, Gorzelak-Leszczynska B. The Polish Gynecological Society recommendations for the detection of group B streptococcus (GBS) carriage in pregnant women and for prevention of neonatal infections caused by this pathogen. Ginekol Pol. 2008;79:221–223. PubMed

Lachenauer CS, Kasper DL, Shimada J, Ichiman Y, Ohtsuka H, Kaku M, Paoletti LC, Ferrieri P, Madoff LC. Serotypes VI and VIII predominate among group B streptococci isolated from pregnant Japanese women. J Infect Dis. 1999;179:1030–1033. doi: 10.1086/314666. PubMed DOI

Lamy MC, Dramsi S, Billoët A, Réglier-Poupet H, Tazi A, Raymond J, Guérin F, Couvé E, Kunst F, Glaser P, Trieu-Cuot P, Poyart C. Rapid detection of the “highly virulent” group B Streptococcus ST-17 clone. Microbes Infect. 2006;8:1714–1722. doi: 10.1016/j.micinf.2006.02.008. PubMed DOI

Law MR, Palomaki G, Alfirevic Z, Gilbert R, Heath P, McCartney C, Reid T, Schrag S. The prevention of neonatal group B streptococcal disease: a report by a working group of the Medical Screening Society. J Med Screen. 2005;12:60–68. doi: 10.1258/0969141053908366. PubMed DOI

Lefevre J, Hankins C, Pourreaux K, Voyer H, Coutlée F, Canadian Women’s HIV Study Group Real-time PCR assays using internal controls for quantitation of HPV-16 and beta-globin DNA in cervicovaginal lavages. J Virol Methods. 2003;114:135–144. doi: 10.1016/j.jviromet.2003.09.003. PubMed DOI

Manning SD, Lewis MA, Springman AC, Lehotzky E, Whittam TS, Davies HD. Genotypic diversity and serotype distribution of group B streptococcus isolated from women before and after delivery. Clin Infect Dis. 2008;46:1829–1837. doi: 10.1086/588296. PubMed DOI PMC

Manning SD, Springman AC, Lehotzky E, Lewis MA, Whittam TS, Davies HD. Multilocus sequence types associated with neonatal group B streptococcal sepsis and meningitis in Canada. J Clin Microbiol. 2009;47:1143–1148. doi: 10.1128/JCM.01424-08. PubMed DOI PMC

Persson E, Berg S, Trollfors B, Larsson P, Ek E, Backhaus E, Claesson BE, Jonsson L, Radberg G, Ripa T, Johansson S. Serotypes and clinical manifestations of invasive group B streptococcal infections in western Sweden 1998–2001. Clin Microbiol Infect. 2004;10:791–796. doi: 10.1111/j.1469-0691.2004.00931.x. PubMed DOI

Pinheiro S, Radhouani H, Coelho C, Gonçalves A, Carvalho E, Carvalho JA, Ruiz-Larrea F, Torres C, Igrejas G, Poeta P. Prevalence and mechanisms of erythromycin resistance in Streptococcus agalactiae from healthy pregnant women. Microb Drug Resist. 2009;15:121–124. doi: 10.1089/mdr.2009.0895. PubMed DOI

Poyart C, Tazi A, Réglier-Poupet H, Billoet A, Tavares N, Raymond J, Trieu-Cuot P. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol. 2007;45:1985–1988. doi: 10.1128/JCM.00159-07. PubMed DOI PMC

Poyart C, Réglier-Poupet H, Tazi A, Billoët A, Dmytruk N, Bidet P, Bingen E, Raymond J, Trieu-Cuot P. Invasive group B streptococcal infections in infants. Fr Emerg Infect Dis. 2008;14:1647–1649. doi: 10.3201/eid1410.080185. PubMed DOI PMC

Slotved HC, Kong F, Lambertsen L, Sauer S, Gilbert GL. Serotype IX, a proposed new Streptococcus agalactiae serotype. J Clin Microbiol. 2007;45:2929–2936. doi: 10.1128/JCM.00117-07. PubMed DOI PMC

Sutcliffe J, Grebe T, Tait-Kamradt A, Wondrack L. Detection of erythromycin-resistant determinants by PCR. Antimicrob Agents Chemother. 1996;40:2562–2566. PubMed PMC

Thigpen MC, Whitney CG, Messonnier NE, Zell ER, Lynfield R, Hadler JL, Harrison LH, Farley MM, Reingold A, Bennett NM, Craig AS, Schaffner W, Thomas A, Lewis MM, Scallan E, Schuchat A. Bacterial meningitis in the United States, 1998–2007. N Engl J Med. 2011;364:2016–2025. doi: 10.1056/NEJMoa1005384. PubMed DOI

Verani J, McGee L, Schrag SJ. Prevention of perinatal group b streptococcal disease revised guidelines from CDC. MMWR Morb Mortal Wkly Rep. 2010;59:1–32. PubMed

Capsule typing and clonal relationships of group B streptococci isolated from male patients diagnosed with urinary tract infection