New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biocatalysis MeSH
- Chromatography, Affinity methods MeSH
- Immobilized Proteins chemistry MeSH
- Immunoglobulin G chemistry MeSH
- Polymethacrylic Acids chemical synthesis MeSH
- Humans MeSH
- Magnets MeSH
- Microspheres MeSH
- Microscopy, Electron, Scanning MeSH
- Polyhydroxyethyl Methacrylate chemical synthesis MeSH
- Polymerization MeSH
- Spectrophotometry, Infrared MeSH
- Trypsin chemistry MeSH
- Ferric Compounds chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ferric oxide MeSH Browser
- Immobilized Proteins MeSH
- Immunoglobulin G MeSH
- Polymethacrylic Acids MeSH
- polyglycidyl methacrylate MeSH Browser
- Polyhydroxyethyl Methacrylate MeSH
- Trypsin MeSH
- Ferric Compounds MeSH
Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.
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