New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22411761
DOI
10.1002/mabi.201100393
Knihovny.cz E-zdroje
- MeSH
- biokatalýza MeSH
- chromatografie afinitní metody MeSH
- imobilizované proteiny chemie MeSH
- imunoglobulin G chemie MeSH
- kyseliny polymethakrylové chemická syntéza MeSH
- lidé MeSH
- magnety MeSH
- mikrosféry MeSH
- mikroskopie elektronová rastrovací MeSH
- polyhydroxyethylmethakrylát chemická syntéza MeSH
- polymerizace MeSH
- spektrofotometrie infračervená MeSH
- trypsin chemie MeSH
- železité sloučeniny chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ferric oxide MeSH Prohlížeč
- imobilizované proteiny MeSH
- imunoglobulin G MeSH
- kyseliny polymethakrylové MeSH
- polyglycidyl methacrylate MeSH Prohlížeč
- polyhydroxyethylmethakrylát MeSH
- trypsin MeSH
- železité sloučeniny MeSH
Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.
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