Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm(2)) and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and explain the cell selectivity.

Institute of Physics Academy of Sciences of the Czech Republic Cukrovarnicka 10 Prague 6 Czech Republic; E Mail ;

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Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

bOptimizing atomic force microscopy for characterization of diamond-protein interfaces

Guided assembly of nanoparticles on electrostatically charged nanocrystalline diamond thin films