Rapid HILIC method with fluorescence detection using derivatization reaction utilizing o-phthaldialdehyde for determination of degradation product of aliskiren
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, validační studie
PubMed
22475518
DOI
10.1016/j.jpba.2012.03.015
PII: S0731-7085(12)00138-0
Knihovny.cz E-zdroje
- MeSH
- amidy analýza chemie MeSH
- aminokyseliny analýza MeSH
- antihypertenziva analýza chemie MeSH
- časové faktory MeSH
- chromatografie kapalinová metody MeSH
- fluorescenční spektrometrie metody MeSH
- fumaráty analýza chemie MeSH
- hmotnostní spektrometrie metody MeSH
- o-ftalaldehyd chemie MeSH
- senzitivita a specificita MeSH
- Publikační typ
- časopisecké články MeSH
- validační studie MeSH
- Názvy látek
- 3-amino-2,2-dimethylpropanamide MeSH Prohlížeč
- aliskiren MeSH Prohlížeč
- amidy MeSH
- aminokyseliny MeSH
- antihypertenziva MeSH
- fumaráty MeSH
- o-ftalaldehyd MeSH
A rapid procedure based on direct extraction and HILIC separation of aliskiren (ALI) degradation product - 3-amino-2,2-dimethylpropanamide (ADPA) with fluorescence detection has been developed. The formation of ADPA from ALI under different conditions was studied. The evaluation of HILIC method robustness was performed using multifactorial experiments with fixed factors (one-level Plackett-Burman design). XBridge HILIC column with isocratic elution using mobile phase 10 mM K(2)HPO(4) pH 7.2-acetonitrile (26:74; v/v) was employed. Fluorescence detection after post column derivatization using o-phthaldialdehyde (OPA) reagent was performed at excitation and emission wavelength of 345 nm and 450 nm, respectively. The reported method has an advantage of a simple sample pre-treatment and quick and very sensitive measurement. The method was successfully applied for the analysis of commercially available ALI samples.
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