The effect of maize silage as co-substrate for swine manure on the bacterial community structure in biogas plants
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- Bacteria klasifikace genetika izolace a purifikace metabolismus MeSH
- biodiverzita MeSH
- biopaliva analýza mikrobiologie MeSH
- bioreaktory mikrobiologie MeSH
- fylogeneze MeSH
- hnůj analýza mikrobiologie MeSH
- kukuřice setá chemie mikrobiologie MeSH
- kultivační média analýza metabolismus MeSH
- molekulární sekvence - údaje MeSH
- siláž analýza mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- biopaliva MeSH
- hnůj MeSH
- kultivační média MeSH
The qualitative and quantitative changes in the bacterial community composition in two mesophilic, commercially used biogas plants were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR. The main objective was to evaluate the influence of the co-substrate maize silage on total bacteria and some selected bacterial groups by comparing full-scale reactors fed solely with pig manure or additionally with maize silage. DGGE fingerprints reflected shifts in the bacterial community structure associated with maize silage as co-substrate and the real-time PCR results showed clear changes in the quantitative composition of the bacterial consortia of each fermenter. A clear dominance of Clostridia in all surveyed fermenters and considerably lower abundance of Bacteroidetes in the biogas plant fed with maize silage was shown.
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