The influence of geographic location, season, age, and part of the digestive tract on bacterial diversity was evaluated on intestinal microflora of honeybees, wasps, and cockroaches using DGGE analysis. PCR-DGGE analyses with universal bacterial primers targeting 200-bp region of the 16S rDNA gene afforded the profile of complex bacterial DNA; specific primers were used to determine the profile of bifidobacteria whose concentration in digestive tract was determined by real-time PCR. Selected PCR products were identified by sequencing. The microflora of the bees exhibited little variations among the hives from distant locations. Their bifidobacterial population formed 2.8-8.4 % of total bacteria and was very homogeneous. The total gut microflora of wasps was also homogeneous, only two samples being affected by the season or the location; on the other hand, wasp bifidobacterial population was very heterogeneous. Cockroaches showed the highest variations in microflora composition, the age and diet being the ultimate factors; bifidobacteria counts also varied among tested individuals (0.1-34.1 % of total bacteria). Our results suggest that nutrition habits are the strongest factor affecting the insect microflora, giving higher variations to omnivorous species.

Institute of Animal Physiology and Genetics v v i Academy of Sciences of the Czech Republic 142 20 Prague Czechia

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Acta Trop. 2005 Mar;93(3):259-65 PubMed

FEMS Microbiol Lett. 2000 Jul 15;188(2):185-90 PubMed

Appl Environ Microbiol. 2003 Nov;69(11):6380-5 PubMed

Insect Mol Biol. 2003 Feb;12(1):85-91 PubMed

FEMS Microbiol Ecol. 2007 Mar;59(3):600-10 PubMed

J Insect Physiol. 2002 Aug;48(8):751-763 PubMed

Nucleic Acids Res. 1994 Sep;22(17):3485-7 PubMed

Biol Lett. 2007 Jun 22;3(3):327-30 PubMed

J Invertebr Pathol. 2003 Oct;84(2):96-103 PubMed

Cell Mol Life Sci. 2002 Jul;59(7):1138-50 PubMed

Appl Environ Microbiol. 1997 Apr;63(4):1268-73 PubMed

Appl Environ Microbiol. 1993 Mar;59(3):695-700 PubMed

Arch Microbiol. 2001 Apr;175(4):259-62 PubMed

Ann Trop Med Parasitol. 2006 Jan;100(1):55-62 PubMed

Proc Natl Acad Sci U S A. 1983 Mar;80(6):1579-83 PubMed

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