Plagiodera versicolora (Laicharting, 1781) (Coleoptera: Chrysomelidae) is an important forest pest which damages many trees such as willow, poplar, and hazelnut. In order to find new microbes that can be utilized as a possible microbial control agent against this pest, we investigated the culturable bacterial flora of it and tested the isolated bacteria against P. versicolora larvae and adults. We were able to isolate nine bacteria from larvae and adults. The isolates were characterized using a combination of morphological, biochemical, and physiological methods. Additionally, we sequenced the partial sequence of the 16S rRNA gene to verify conventional identification results. Based on characterization studies, the isolates were identified as Staphylococcus sp. Pv1, Rahnella sp. Pv2, Rahnella sp. Pv3, Rahnella sp. Pv4, Rahnella sp. Pv5, Pantoea agglomerans Pv6, Staphylococcus sp. Pv7, Micrococcus luteus Pv8, and Rahnella sp. Pv9. The highest insecticidal activity against larvae and adults was obtained from M. luteus Pv8 with 50 and 40 % mortalities within 10 days after treatment, respectively. Extracellular enzyme activity of the bacterial isolates such as amylase, proteinase, lipase, cellulose, and chitinase was also determined. Consequently, our results show that M. luteus Pv8 might be a good candidate as a possible microbial control agent against P. versicolora and were discussed with respect to biocontrol potential of the bacterial isolates.

Department of Biology Faculty of Arts and Sciences Recep Tayyip Erdoğan University Rize 53100 Turkey

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J Invertebr Pathol. 2002 May;80(1):7-12 PubMed

J Microbiol. 2005 Oct;43(5):463-8 PubMed

Int J Syst Bacteriol. 1998 Jan;48 Pt 1:141-9 PubMed

Nucleic Acids Res. 2012 Jan;40(Database issue):D48-53 PubMed

Curr Microbiol. 2001 Apr;42(4):290-4 PubMed

Appl Environ Microbiol. 2011 Jul;77(13):4669-75 PubMed

J Clin Microbiol. 2007 Jun;45(6):1989-92 PubMed

Appl Environ Microbiol. 2001 Sep;67(9):3852-9 PubMed

Acta Microbiol Pol A. 1972;4(3):127-40 PubMed

Am J Trop Med Hyg. 1992 Feb;46(2):195-200 PubMed

J Gen Appl Microbiol. 1997 Dec;43(6):363-367 PubMed

J Insect Sci. 2010;10:107 PubMed

World J Microbiol Biotechnol. 1992 Jul;8(4):451-2 PubMed

J Mol Biol. 1990 Oct 5;215(3):403-10 PubMed

Curr Microbiol. 2009 May;58(5):478-82 PubMed

Folia Microbiol (Praha). 2008;53(3):229-33 PubMed

J Microbiol Biotechnol. 2012 Jan;22(1):133-40 PubMed

Nature. 2000 Feb 24;403(6772):851 PubMed

J Appl Microbiol. 1999 Apr;86(4):622-34 PubMed

J Clin Microbiol. 1989 Jul;27(7):1671-2 PubMed

J Bacteriol. 1995 May;177(10):2851-7 PubMed

Proc Natl Acad Sci U S A. 1981 May;78(5):2893-7 PubMed

Mol Biol Evol. 1987 Jul;4(4):406-25 PubMed

Mol Biol Evol. 2011 Oct;28(10):2731-9 PubMed

Annu Rev Entomol. 2004;49:71-92 PubMed

Appl Environ Microbiol. 1982 Apr;43(4):777-80 PubMed

J Appl Microbiol. 2001 Jun;90(6):850-8 PubMed

Curr Microbiol. 2008 Apr;56(4):391-6 PubMed

Emerg Infect Dis. 1998 Oct-Dec;4(4):581-91 PubMed

Biosci Biotechnol Biochem. 2002 Nov;66(11):2474-8 PubMed

Evolution. 1985 Jul;39(4):783-791 PubMed

Folia Microbiol (Praha). 2010 Jan;55(1):35-8 PubMed

Phytopathology. 1998 Nov;88(11):1149-57 PubMed

J Basic Microbiol. 2012 Dec;52(6):695-704 PubMed

Appl Environ Microbiol. 1987 Jan;53(1):211-3 PubMed

Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PubMed