Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens
Jazyk angličtina Země Švýcarsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22540115
PII: 2011_220
Knihovny.cz E-zdroje
- MeSH
- bilirubin analogy a deriváty izolace a purifikace metabolismus MeSH
- chromatografie na tenké vrstvě MeSH
- Clostridium perfringens izolace a purifikace metabolismus MeSH
- feces mikrobiologie MeSH
- hydrolýza MeSH
- lidé MeSH
- novorozenec MeSH
- oxidace-redukce MeSH
- střeva mikrobiologie MeSH
- taurin analogy a deriváty izolace a purifikace metabolismus MeSH
- urobilinogen MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bilirubin ditaurine MeSH Prohlížeč
- bilirubin MeSH
- taurin MeSH
- urobilinogen MeSH
Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria.
