Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22540115
PII: 2011_220
Knihovny.cz E-resources
- MeSH
- Bilirubin analogs & derivatives isolation & purification metabolism MeSH
- Chromatography, Thin Layer MeSH
- Clostridium perfringens isolation & purification metabolism MeSH
- Feces microbiology MeSH
- Hydrolysis MeSH
- Humans MeSH
- Infant, Newborn MeSH
- Oxidation-Reduction MeSH
- Intestines microbiology MeSH
- Taurine analogs & derivatives isolation & purification metabolism MeSH
- Urobilinogen MeSH
- Check Tag
- Humans MeSH
- Infant, Newborn MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- bilirubin ditaurine MeSH Browser
- Bilirubin MeSH
- Taurine MeSH
- Urobilinogen MeSH
Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria.
