Impact of normalization method on experimental outcome using RT-qPCR in Staphylococcus aureus
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22613804
DOI
10.1016/j.mimet.2012.05.008
PII: S0167-7012(12)00186-8
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial * MeSH
- RNA, Bacterial genetics MeSH
- DNA Gyrase genetics MeSH
- Gene Expression MeSH
- Real-Time Polymerase Chain Reaction standards MeSH
- Reverse Transcriptase Polymerase Chain Reaction standards MeSH
- Reference Standards MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Staphylococcus aureus genetics MeSH
- Transcriptome MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Bacterial MeSH
- DNA Gyrase MeSH
- RNA, Ribosomal, 16S MeSH
We performed a gene expression study using RT-qPCR in Staphylococcus aureus. The influence of normalization method was investigated. We confirmed that a recent standard, using more reference genes, was the best normalization strategy. The application of the most commonly used reference genes in 2011 (gyrB and 16S rRNA gene) failed.
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