Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22644213
DOI
10.1039/c2cc32930a
Knihovny.cz E-resources
- MeSH
- Deoxycytosine Nucleotides chemistry MeSH
- DNA Primers metabolism MeSH
- DNA Breaks, Single-Stranded MeSH
- Oligonucleotides biosynthesis MeSH
- Deoxyribonucleases, Type II Site-Specific metabolism MeSH
- Nucleic Acid Amplification Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 2'-deoxycytidine 5'-triphosphate MeSH Browser
- Deoxycytosine Nucleotides MeSH
- DNA Primers MeSH
- endodeoxyribonuclease BstNBI MeSH Browser
- Oligonucleotides MeSH
- Deoxyribonucleases, Type II Site-Specific MeSH
A method for enzymatic production of short (10-20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers.
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