Double-headed sulfur-linked amino acids as first inhibitors for betaine-homocysteine S-methyltransferase 2
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22775318
DOI
10.1021/jm300571h
Knihovny.cz E-resources
- MeSH
- Betaine-Homocysteine S-Methyltransferase antagonists & inhibitors chemistry MeSH
- Enzyme Assays MeSH
- Homocysteine analogs & derivatives chemical synthesis chemistry MeSH
- Kinetics MeSH
- Humans MeSH
- Recombinant Proteins antagonists & inhibitors MeSH
- Stereoisomerism MeSH
- Sulfides chemical synthesis chemistry MeSH
- Structure-Activity Relationship MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 5-thia-2,11-diamino-8-methyldodecanedioic acid MeSH Browser
- Betaine-Homocysteine S-Methyltransferase MeSH
- BHMT2 protein, human MeSH Browser
- Homocysteine MeSH
- Recombinant Proteins MeSH
- Sulfides MeSH
Betaine-homocysteine S-methyltransferase 2 (BHMT-2) catalyzes the transfer of a methyl group from S-methylmethionine to l-homocysteine, yielding two molecules of l-methionine. It is one of three homocysteine methyltransferases in mammals, but its overall contribution to homocysteine remethylation and sulfur amino acid homeostasis is not known. Moreover, recombinant BHMT-2 is highly unstable, which has slowed research on its structural and catalytic properties. In this study, we have prepared the first series of BHMT-2 inhibitors to be described, and we have tested them with human recombinant BHMT-2 that has been stabilized by copurification with human recombinant BHMT. Among the compounds synthesized, (2S,8RS,11RS)-5-thia-2,11-diamino-8-methyldodecanedioic acid (11) was the most potent (K(i)(app) ∼77 nM) and selective inhibitor of BHMT-2. Compound 11 only weakly inhibited human BHMT (IC(50) about 77 μM). This compound (11) may be useful in future in vivo studies to probe the physiological significance of BHMT-2 in sulfur amino acid metabolism.
J Med Chem. 2012 Oct 25;55(20):8974 PubMed
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