An influence of ethanol and temperature on products formation by different preparations of Zymomonas mobilis extracellular levansucrase
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Ethanol pharmacology MeSH
- Hexosyltransferases metabolism MeSH
- Hydrogen-Ion Concentration MeSH
- Oligosaccharides analysis biosynthesis MeSH
- Temperature MeSH
- Zymomonas enzymology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ethanol MeSH
- fructooligosaccharide MeSH Browser
- Hexosyltransferases MeSH
- levansucrase MeSH Browser
- Oligosaccharides MeSH
The ethanol and temperature effects on the ratio between Zymomonas mobilis 113S extracellular levansucrase activities were studied using fermentation broth supernatant, "levan-levansucrase" sediment precipitated by ethanol and highly purified enzyme. The fructooligosaccharide (FOS) production at different temperatures in the presence of ethanol was investigated. An ethanol increases FOS biosynthesis activity part of levansucrase. Especially, this effect was pronounced at lower temperatures (35-40 °C) and using purified levansucrase. The inverse relationship between temperature and ratio synthetic activity/total activity of levansucrase was found. The FOS composition containing mostly 1-kestose, 6-kestose, and neokestose obtained in the presence of different ethanol concentrations was found relative constant, while the changes in the sucrose concentration and temperature gave slight changes in the ratio between 1-kestose and 6-kestose.
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