Microbial control of the cotton leafworm Spodoptera littoralis (Boisd.) by Egyptian Bacillus thuringiensis isolates
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- analýza přežití MeSH
- Bacillus thuringiensis klasifikace genetika izolace a purifikace patogenita MeSH
- bakteriální proteiny biosyntéza genetika toxicita MeSH
- biotest MeSH
- DNA bakterií chemie genetika MeSH
- endotoxiny biosyntéza genetika toxicita MeSH
- Gossypium parazitologie MeSH
- hemolyziny biosyntéza genetika toxicita MeSH
- larva účinky léků MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce MeSH
- sekvenční analýza DNA MeSH
- Spodoptera mikrobiologie fyziologie MeSH
- toxiny Bacillus thuringensis MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Egypt MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- endotoxiny MeSH
- hemolyziny MeSH
- insecticidal crystal protein, Bacillus Thuringiensis MeSH Prohlížeč
- toxiny Bacillus thuringensis MeSH
Four local Bacillus thuringiensis (Bt) isolates that had been serologically identified as Bt var. kurstaki (Btk2, Btk3, and Btk66) and Bt var. mexicanensis (Btm27), in addition to two reference strains (4D20 and 4AC1), were laboratory assayed as microbial control agents against the Egyptian cotton leafworm Spodoptera littoralis (Boisd.). Polymerase chain reaction (PCR) amplification analysis revealed that each of the six experimental strains carries, at least, a cry1 type gene which expresses a protein toxin active against lepidopterous insects. Additionally, PCR amplification results demonstrated that 4D20 and Btk66 contain the Lepidoptera- and Diptera-active cry2 type gene and that Btk66 contains Coleoptera-active cry7 and cry8 genes. Among the six strains, Btk66 and Btm27 were the most promising microbial control agents against S. littoralis. The present findings were the first to report that Btm27 (classified as B. thuringiensis var. mexicanensis) is a very potent microbial control agent against S. littoralis-tested larvae. For more characterization of these two isolates, the sspO gene was investigated as a molecular chronometer. The DNA sequencing results proved that Btk66 and Btm27 carry sspO open reading frames with identical nucleotide sequences, suggesting a strong phylogenetic relationship between the two strains.
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Cry3Aa Toxin Is Not Suitable to Control Lepidopteran Pest Spodoptera littoralis (Boisd.)
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