Characterization of IncN plasmids carrying bla CTX-M-1 and qnr genes in Escherichia coli and Salmonella from animals, the environment and humans
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23060365
DOI
10.1093/jac/dks387
PII: dks387
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial MeSH
- Bacterial Infections microbiology veterinary MeSH
- beta-Lactamases genetics MeSH
- DNA, Bacterial genetics MeSH
- Escherichia coli drug effects genetics isolation & purification MeSH
- Nucleic Acid Hybridization MeSH
- Humans MeSH
- Environmental Microbiology * MeSH
- Molecular Sequence Data MeSH
- Multilocus Sequence Typing MeSH
- Plasmids * MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Escherichia coli Proteins genetics MeSH
- Salmonella drug effects genetics isolation & purification MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Europe MeSH
- Names of Substances
- beta-lactamase TEM-3 MeSH Browser
- beta-Lactamases MeSH
- DNA, Bacterial MeSH
- Escherichia coli Proteins MeSH
- Qnr protein, E coli MeSH Browser
OBJECTIVES: The aim of the study was to characterize a collection of Escherichia coli and Salmonella harbouring qnr and bla(CTX-M-1) genes on IncN plasmids isolated from humans, food-producing, companion and wild animals, and the environment from six European countries. METHODS: Nineteen IncN plasmids were compared using restriction fragment length polymorphism (RFLP), plasmid multilocus sequence typing (pMLST) and hybridization with repN, qnrS1, qnrB19 or bla(CTX-M-1) probes. Plasmids pKT58A and pHHA45 were sequenced using the 454-Genome Sequencer FLX platform on a library constructed from plasmid DNA purified from the respective E. coli transformants. RESULTS: Three types of IncN plasmids carrying bla(CTX-M-1), qnrS1 and qnrB19 genes were identified in strains isolated from the Czech Republic, Poland, Slovakia, Denmark, Italy and the Netherlands, corresponding to pMLST sequence type (ST) 1, ST3 and ST8, respectively. Related plasmids circulating in human and animal isolates were identified. Complete nucleotide sequences of the ST1 pHHA45 plasmid carrying bla(CTX-M-1), isolated from E. coli from pigs in Denmark, and the ST3 pKT58A plasmid harbouring qnrS1, identified in E. coli from a water bird, were obtained. CONCLUSIONS: Our results demonstrated wide distribution of specific IncN plasmids disseminating bla(CTX-M-1) and qnr genes among animals and humans in Europe.
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