Chalicogloea cavernicola gen. nov., sp. nov. (Chroococcales, Cyanobacteria), from low-light aerophytic environments: combined molecular, phenotypic and ecological criteria
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23178723
DOI
10.1099/ijs.0.045468-0
Knihovny.cz E-zdroje
- MeSH
- chlorofyl a MeSH
- chlorofyl analýza MeSH
- DNA bakterií genetika MeSH
- fykocyanin analýza MeSH
- fykoerythrin analýza MeSH
- fylogeneze * MeSH
- jeskyně mikrobiologie MeSH
- konfokální mikroskopie MeSH
- molekulární sekvence - údaje MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- sinice klasifikace genetika izolace a purifikace MeSH
- transmisní elektronová mikroskopie MeSH
- tylakoidy ultrastruktura MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Španělsko MeSH
- Názvy látek
- allophycocyanin MeSH Prohlížeč
- chlorofyl a MeSH
- chlorofyl MeSH
- DNA bakterií MeSH
- fykocyanin MeSH
- fykoerythrin MeSH
- RNA ribozomální 16S MeSH
This work characterizes a unicellular cyanobacterium with nearly spherical cells and thin-outlined sheaths that divide irregularly, forming small packets immersed in a diffluent mucilaginous layer. It was isolated growing on calcite speleothems and walls in a show cave in Collbató (Barcelona, Spain). Spectral confocal laser and transmission electron microscopy were used to describe the morphology, fine structure and thylakoid arrangement. The pigments identified were phycoerythrin, phycocyanin, allophycocyanin and chlorophyll a. Three-dimensional reconstructions, generated from natural fluorescence z-stacks, revealed a large surface area of nearly flat, arm-like thylakoidal membranes connected to each other and forming a unified structure in a way that, to our knowledge, has never been described before. Phylogenetic analyses using the 16S rRNA gene sequence showed 95% similarity to strain Chroococcus sp. JJCM (GenBank accession no. AM710384). The diacritical phenotypic features do not correspond to any species currently described, and the genetic traits support the strain being classified as the first member of an independent genus in the order Chroococcales and the family Chroococcaceae. Hence, we propose the name Chalicogloea cavernicola gen. nov., sp. nov. under the provisions of the International Code of Nomenclature for Algae, Fungi and Plants. The type strain of Chalicogloea cavernicola is COLL 3(T) (=CCALA 975(T) =CCAP 1424/1(T)).
Citace poskytuje Crossref.org
Four novel taxa of cyanobacteria from a unique thermal cave habitat in Vromoner Canyon, Albania
GENBANK
JQ967037
