In vitro transfection mediated by dendrigraft poly(L-lysines): the effect of structure and molecule size
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Line MeSH
- Deoxyribonuclease I metabolism MeSH
- Humans MeSH
- Lipids MeSH
- RNA, Small Interfering MeSH
- Molecular Sequence Data MeSH
- Oligonucleotides metabolism pharmacokinetics MeSH
- Plasmids pharmacokinetics MeSH
- Polylysine chemistry pharmacokinetics MeSH
- Base Sequence MeSH
- Transfection methods MeSH
- Cell Survival drug effects MeSH
- Structure-Activity Relationship MeSH
- Green Fluorescent Proteins genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Deoxyribonuclease I MeSH
- enhanced green fluorescent protein MeSH Browser
- Lipids MeSH
- Lipofectamine MeSH Browser
- RNA, Small Interfering MeSH
- Oligonucleotides MeSH
- Polylysine MeSH
- Green Fluorescent Proteins MeSH
Dendritic poly(L-lysines) (DGL) constitute promising nanomaterials applicable as a nonviral gene-delivery vector. In this study, we evaluate the transfection abilities of four DGL generations with special emphasis on the systematic description of the relationship of how generation (i.e., molecule size) affects the transfection efficacy. Using Hep2 cells, we demonstrated that the capability of unmodified DGL to deliver plasmid is of a magnitude lower than that of jetPEI. On the other hand, employing the Hep2 cell line stably transduced with eGFP, we observed that DGL G5 delivers the siRNA oligonucleotide with the same efficiency as Lipofectamine 2000. In further experiments, it was shown that DGL affords excellent ability to bind DNA, protect it against DNase I attack, and internalize it into cells.
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