Immunogenomic analysis of insect bite hypersensitivity in a model horse population
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23351640
DOI
10.1016/j.vetimm.2012.12.013
PII: S0165-2427(12)00450-3
Knihovny.cz E-resources
- MeSH
- Allergens immunology MeSH
- Dermatitis, Atopic genetics immunology veterinary MeSH
- Ceratopogonidae immunology MeSH
- Cytokines genetics MeSH
- Gene Expression MeSH
- Major Histocompatibility Complex MeSH
- Immunoglobulin E blood MeSH
- Interferon-gamma genetics MeSH
- Janus Kinase 2 genetics MeSH
- Polymorphism, Single Nucleotide MeSH
- Horses MeSH
- Insect Bites and Stings genetics immunology veterinary MeSH
- RNA, Messenger genetics MeSH
- Horse Diseases genetics immunology MeSH
- Protein Precursors genetics MeSH
- Salivary Proteins and Peptides immunology MeSH
- Thymic Stromal Lymphopoietin MeSH
- Transforming Growth Factor beta1 genetics MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Allergens MeSH
- Cytokines MeSH
- Immunoglobulin E MeSH
- Interferon-gamma MeSH
- involucrin MeSH Browser
- Janus Kinase 2 MeSH
- RNA, Messenger MeSH
- Protein Precursors MeSH
- Salivary Proteins and Peptides MeSH
- Thymic Stromal Lymphopoietin MeSH
- Transforming Growth Factor beta1 MeSH
Equine insect bite hypersensitivity (IBH) is a seasonal IgE-mediated dermatosis caused by bites of insects of the genus Culicoides. A familial predisposition for the disease has been shown but, except for the MHC, the genes involved have not been identified so far. An immunogenomic analysis of IBH was performed in a model population of Old Kladruby horses, all living in the same environment. Clinical signs of IBH were used as phenotypic manifestation of IBH. Furthermore, total serum IgE levels were determined in the sera of these horses and used as an independent phenotypic marker for the immunogenetic analysis. Single nucleotide polymorphisms (SNPs) in candidate immunity-related genes were used for association analyses. Genotypes composed of two to five genes encoding interferon gamma -IFNG, transforming growth factor beta 1 -TGFB1, Janus kinase 2 -JAK2, thymic stromal lymphopoietin -TSLP, and involucrin -IVL were associated with IBH, indicating a role of the genes in the pathogenesis of IBH. These findings were supported by analysis of gene expression in skin biopsies of 15 affected and 15 unaffected horses. Two markers associated with IBH, IFNG and TGFB1, showed differences in mRNA expression in skin biopsies from IBH-affected and non-affected horses (p<0.05). Expression of the gene coding for the CD14 receptor molecule -CD14 was different in skin biopsies at p<0.06. When total IgE levels were treated as binary traits, genotypes of IGHE, ELA-DRA, and IL10/b were associated with this trait. When treated as a continuous trait, total IgE levels were associated with genes IGHE, FCER1A, IL4, IL4R, IL10, IL1RA, and JAK2. This first report on non-MHC genes associated with IBH in horses is thus supported by differences in expression of genes known to play a role in allergy and immunity.
References provided by Crossref.org
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