Comparison of myosin heavy chain mRNAs, protein isoforms and fiber type proportions in the rat slow and fast muscles
Language English Country Czech Republic Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
23590611
DOI
10.33549/physiolres.932418
PII: 932418
Knihovny.cz E-resources
- MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Immunohistochemistry MeSH
- Rats MeSH
- Real-Time Polymerase Chain Reaction MeSH
- RNA, Messenger metabolism MeSH
- Rats, Inbred Lew MeSH
- Protein Isoforms MeSH
- Gene Expression Regulation MeSH
- Muscle Fibers, Slow-Twitch metabolism MeSH
- Muscle Fibers, Fast-Twitch metabolism MeSH
- Myosin Heavy Chains genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- RNA, Messenger MeSH
- Protein Isoforms MeSH
- Myosin Heavy Chains MeSH
We studied the expression of myosin heavy chain isoforms at mRNA and protein levels as well as fiber type composition in the fast extensor digitorum longus (EDL) and slow soleus (SOL) twitch muscles of adult inbred Lewis strain rats. Comparison of the results from Real Time RT-PCR, SDS-PAGE and fiber type analysis showed corresponding proportions of MyHC transcripts (MyHC-1, -2a, -2x/d, -2b), protein isoforms (MyHC-1, -2a, -2x/d, -2b) and fiber types (type 1, 2A, 2X/D, 2B) in both muscles. Furthermore, we found that slow MyHC-1 mRNA expression in the SOL was up to three orders higher than that of fast MyHC transcripts. This finding can explain the predominance of MyHC-1 isoform and fiber type 1 and the absence of pure 2X/D and 2B fibers in the SOL muscle. Based on our data presenting quantitative evidence of corresponding proportions between mRNA level, protein content and fiber type composition, we suggest that the Real Time RT-PCR technique can be used as a routine method for analysis of muscle composition changes and could be advantageous for the analysis of scant biological samples such as muscle biopsies in humans.
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