Alkaloid chelirubine and DNA: blue and red luminescence
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23598024
DOI
10.1016/j.talanta.2012.10.045
PII: S0039-9140(12)00876-4
Knihovny.cz E-resources
- MeSH
- Alkaloids chemistry MeSH
- DNA chemistry MeSH
- Luminescence MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alkaloids MeSH
- DNA MeSH
Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new utilization for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by near-UV light. Besides quantification of DNA (LOD = 6 ng ml(-1)) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter into the living cell through the cell membrane.
Department of Chemistry Faculty of Science Masaryk University Kotlarska 2 Brno 611 37 Czech Republic
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