Alkaloid chelirubine and DNA: blue and red luminescence
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23598024
DOI
10.1016/j.talanta.2012.10.045
PII: S0039-9140(12)00876-4
Knihovny.cz E-zdroje
- MeSH
- alkaloidy chemie MeSH
- DNA chemie MeSH
- luminiscence MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkaloidy MeSH
- DNA MeSH
Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new utilization for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by near-UV light. Besides quantification of DNA (LOD = 6 ng ml(-1)) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter into the living cell through the cell membrane.
Department of Chemistry Faculty of Science Masaryk University Kotlarska 2 Brno 611 37 Czech Republic
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