Method for the determination of thyreostats in milk samples using LC-MS/MS
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Validation Study
- MeSH
- Chromatography, Liquid methods MeSH
- Milk chemistry MeSH
- Tandem Mass Spectrometry methods MeSH
- Antithyroid Agents analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Validation Study MeSH
- Names of Substances
- Antithyroid Agents MeSH
A sensitive and selective analytical method for the determination of four thyreostats (tapazol, thiouracil, methylthiouracil and propylthiouracil) in cow's milk, lamb's milk, and goat's milk was developed and validated according to 2002/657/EC criteria. Proteins in milk samples were precipitated by acetonitrile and analytes were derivatised with 3-iodobenzylbromide. Afterwards, derivatives were separated from the matrix by liquid-liquid extraction with ethyl acetate as an organic solvent and analysis was carried out using LC-MS/MS in a positive electrospray mode. The method provides, for all determined analytes, decision limits CCα below 1 ng ml(-1) and a detection capability CCβ value below 1.5 ng ml(-1). The stability of analytes in sample extracts stored at various conditions was also tested and evaluated.
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