Effects of anthocyanins on the AhR-CYP1A1 signaling pathway in human hepatocytes and human cancer cell lines
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
Grantová podpora
P42 ES004699
NIEHS NIH HHS - United States
ES04699
NIEHS NIH HHS - United States
Odkazy
PubMed
23735880
PubMed Central
PMC3759295
DOI
10.1016/j.toxlet.2013.05.007
PII: S0378-4274(13)00207-5
Knihovny.cz E-zdroje
- Klíčová slova
- 2,3,7,8-tetrachlorodibenzo-p-dioxin, AhR, Anthocyanins, Aryl hydrocarbon receptor, Cytochrome P450, Food supplements, Food–drug interactions, TCDD, aryl hydrocarbon receptor,
- MeSH
- anthokyaniny chemie toxicita MeSH
- buňky Hep G2 MeSH
- cytochrom P-450 CYP1A1 metabolismus MeSH
- dospělí MeSH
- glukosidy chemie toxicita MeSH
- hepatocyty účinky léků metabolismus MeSH
- inhibitory enzymů toxicita MeSH
- interakce mezi potravou a léky MeSH
- jaterní mikrozomy účinky léků enzymologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- potravní doplňky MeSH
- receptory aromatických uhlovodíků účinky léků metabolismus MeSH
- regulace genové exprese enzymů účinky léků MeSH
- signální transdukce účinky léků MeSH
- vazba proteinů MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- anthokyaniny MeSH
- cyanidin-3-O-beta-glucopyranoside MeSH Prohlížeč
- cytochrom P-450 CYP1A1 MeSH
- glukosidy MeSH
- inhibitory enzymů MeSH
- pelargonidin MeSH Prohlížeč
- receptory aromatických uhlovodíků MeSH
Anthocyanins are plant pigments occurring in flowers and berry fruits. Since a phenomenon of food-drug interactions is increasingly emerging, we examined the effects of 21 major anthocyanins and the extracts from 3 food supplements containing anthocyanins on the aryl hydrocarbon receptor (AhR)-cytochrome P450 CYP1A1 signaling pathway in human hepatocytes and human hepatic HepG2 and intestinal LS174T cancer cells. Pelargonidin-3-O-rutinoside (PEL-2) and cyanidin-3,5-O-diglucoside (CYA-3) dose-dependently activated AhR, as revealed by gene reporter assay. PEL-2 and CYA-3 induced CYP1A1 mRNA but not protein in HepG2 and LS174T cells. Neither compounds induced CYP1A1 mRNA and protein in four different primary human hepatocytes cultures. The effects of PEL-2 and CYA-3 on AhR occurred by ligand-dependent and ligand-independent mechanisms, respectively, as demonstrated by ligand binding assay. In a direct enzyme inhibition assay, none of the antocyanins tested inhibited the CYP1A1 marker activity to less than 50% even at 100 μM concentration. PEL-2 and CYA-3 at 100 μM inhibited CYP1A1 to 79% and 65%, respectively. In conclusion, with exception of PEL-2 and CYA-3, there were no effects of 19 major anthocyanins and 3 food supplements containing anthocyanins on AhR-CYP1A1 signaling, implying zero potential of these compounds for food-drug interactions with respect to AhR-CYP1A1 pathway.
Durst RW, WRE . Separation and characterization of anthocyanins by HPLC. In: Wrolstad REATE, Decker EA, Penner MH, Reid DS, Schwartz SJ, Shoemaker CF, Smith DM, Sporns P, editors. Current Protocols in Food Analytical Chemistry. vol Unit F1.3. New York: Wiley; 2001. pp. F1.3.1–F1.3.13.