Low-cost, easy-to-prepare magnetic chitosan microparticles for enzymes immobilization

. 2013 Jul 25 ; 96 (2) : 545-8. [epub] 20130418

Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid23768599
Odkazy

PubMed 23768599
DOI 10.1016/j.carbpol.2013.04.014
PII: S0144-8617(13)00367-6
Knihovny.cz E-zdroje

Extremely simple procedures for the preparation of magnetic chitosan enzyme carriers have been developed and used for the immobilization of lipase and β-galactosidase as model enzymes. In the first case commercially available magnetite microparticles were entrapped in chitosan gel, while in the second case magnetic iron oxides microparticles were synthesized in chitosan matrix from ferrous sulfate using microwave irradiation. Immobilized enzymes showed long-term stability without leaching of enzyme from the support and enabled their repeated use without significant loss of activity.

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