Human embryonic and induced pluripotent stem cells express TRAIL receptors and can be sensitized to TRAIL-induced apoptosis
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23806100
PubMed Central
PMC3822377
DOI
10.1089/scd.2013.0057
Knihovny.cz E-zdroje
- MeSH
- antigeny CD95 genetika metabolismus MeSH
- apoptóza účinky léků MeSH
- buněčná diferenciace MeSH
- embryonální kmenové buňky cytologie účinky léků metabolismus MeSH
- FLIP (buněčný) antagonisté a inhibitory genetika metabolismus MeSH
- harringtoniny farmakologie MeSH
- homoharringtonin MeSH
- inhibitory syntézy proteinů farmakologie MeSH
- kaspasa 3 genetika metabolismus MeSH
- kaspasa 8 genetika metabolismus MeSH
- lidé MeSH
- malá interferující RNA genetika metabolismus MeSH
- pluripotentní kmenové buňky cytologie účinky léků metabolismus MeSH
- proliferace buněk MeSH
- protein MCL-1 genetika metabolismus MeSH
- protein TRAIL genetika metabolismus farmakologie MeSH
- receptory TNF - typ I genetika metabolismus MeSH
- regulace genové exprese MeSH
- signální transdukce MeSH
- synergismus léků MeSH
- TRAIL receptory genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny CD95 MeSH
- FAS protein, human MeSH Prohlížeč
- FLIP (buněčný) MeSH
- harringtoniny MeSH
- homoharringtonin MeSH
- inhibitory syntézy proteinů MeSH
- kaspasa 3 MeSH
- kaspasa 8 MeSH
- malá interferující RNA MeSH
- MCL1 protein, human MeSH Prohlížeč
- protein MCL-1 MeSH
- protein TRAIL MeSH
- receptory TNF - typ I MeSH
- TNFRSF10A protein, human MeSH Prohlížeč
- TNFSF10 protein, human MeSH Prohlížeč
- TRAIL receptory MeSH
Death ligands and their tumor necrosis factor receptor (TNFR) family receptors are the best-characterized and most efficient inducers of apoptotic signaling in somatic cells. In this study, we analyzed whether these prototypic activators of apoptosis are also expressed and able to be activated in human pluripotent stem cells. We examined human embryonic stem cells (hESC) and human-induced pluripotent stem cells (hiPSC) and found that both cell types express primarily TNF-related apoptosis-inducing ligand (TRAIL) receptors and TNFR1, but very low levels of Fas/CD95. We also found that although hESC and hiPSC contain all the proteins required for efficient induction and progression of extrinsic apoptotic signaling, they are resistant to TRAIL-induced apoptosis. However, both hESC and hiPSC can be sensitized to TRAIL-induced apoptosis by co-treatment with protein synthesis inhibitors such as the anti-leukemia drug homoharringtonine (HHT). HHT treatment led to suppression of cellular FLICE inhibitory protein (cFLIP) and Mcl-1 expression and, in combination with TRAIL, enhanced processing of caspase-8 and full activation of caspase-3. cFLIP likely represents an important regulatory node, as its shRNA-mediated down-regulation significantly sensitized hESC to TRAIL-induced apoptosis. Thus, we provide the first evidence that, irrespective of their origin, human pluripotent stem cells express canonical components of the extrinsic apoptotic system and on stress can activate death receptor-mediated apoptosis.
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