Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis
Jazyk angličtina Země Švýcarsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23888296
PII: 2012_368
Knihovny.cz E-zdroje
- MeSH
- Agrobacterium tumefaciens genetika MeSH
- Bacillus thuringiensis genetika MeSH
- bakteriální proteiny genetika MeSH
- endotoxiny genetika MeSH
- genetická transkripce MeSH
- genetické vektory MeSH
- geneticky modifikované rostliny * MeSH
- hemolyziny genetika MeSH
- jedle embryologie genetika MeSH
- promotorové oblasti (genetika) MeSH
- semena rostlinná genetika růst a vývoj MeSH
- somatická embryogeneze rostlin MeSH
- toxiny Bacillus thuringensis MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- endotoxiny MeSH
- hemolyziny MeSH
- insecticidal crystal protein, Bacillus Thuringiensis MeSH Prohlížeč
- toxiny Bacillus thuringensis MeSH
Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.
