Determination of asymmetric and symmetric dimethylarginines in human plasma by HPLC with electrochemical detection
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, validační studie
PubMed
24115463
DOI
10.1002/jssc.201300813
Knihovny.cz E-zdroje
- Klíčová slova
- Arginine, Asymmetric dimethylarginine, Electrochemical detection, HPLC, Symmetric dimethylarginine,
- MeSH
- arginin analogy a deriváty krev MeSH
- biochemická analýza krve přístrojové vybavení metody MeSH
- elektrochemické techniky * MeSH
- lidé MeSH
- limita detekce MeSH
- referenční standardy MeSH
- vysokoúčinná kapalinová chromatografie * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- validační studie MeSH
- Názvy látek
- arginin MeSH
- dimethylarginine MeSH Prohlížeč
A new HPLC method was developed and validated for the determination of asymmetric and symmetric dimethylarginines and l-arginine in human plasma. After SPE and evaporation of the eluate, the samples were derivatised with an o-phthaldialdehyde reagent containing 3-mercaptopropionic acid. The derivatives formed were analysed by isocratic RP-HPLC with electrochemical detection at +320 mV. The mobile phase consisted of 50 mM phosphate buffer (pH 6.1) containing 10% v/v acetonitrile, the flow rate was 1 mL/min. The retention times of all compounds including monomethylarginine (internal standard) were <24 min. The LODs (S/N 3:1) were 0.012 μM for both dimethylarginines and 0.013 μM for L-arginine; the linearity of the method was from 0.1 to 20 μM for both dimethylarginines and from 1 to 200 μM for L-arginine. Absolute extraction recoveries measured for all analytes ranged from 85 to 88%.
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