Effects of artificial sweeteners on the AhR- and GR-dependent CYP1A1 expression in primary human hepatocytes and human cancer cells
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24120730
DOI
10.1016/j.tiv.2013.10.001
PII: S0887-2333(13)00256-7
Knihovny.cz E-resources
- Keywords
- 2,3,7,8-tetrachlorodibenzo-p-dioxin, AhR, Artificial sweeteners, Aryl hydrocarbon Receptor, Aryl hydrocarbon receptor, CAR, Constitutive androstane receptor, Cytochrome P450, ER, Food–drug interactions, GR, Glucocorticoid receptor, LXR, PXR, RXR, TCDD, estrogen receptor, glucocorticoid receptor, liver X receptor, pregnane X receptor, retinoic X receptor,
- MeSH
- Aspartame pharmacology MeSH
- Cyclamates pharmacology MeSH
- Cytochrome P-450 CYP1A1 genetics metabolism MeSH
- Hepatocytes drug effects metabolism MeSH
- Food-Drug Interactions * MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Receptors, Aryl Hydrocarbon metabolism MeSH
- Receptors, Glucocorticoid metabolism MeSH
- Saccharin pharmacology MeSH
- Sweetening Agents pharmacology MeSH
- Thiazines pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- acetosulfame MeSH Browser
- Aspartame MeSH
- Cyclamates MeSH
- CYP1A1 protein, human MeSH Browser
- Cytochrome P-450 CYP1A1 MeSH
- Receptors, Aryl Hydrocarbon MeSH
- Receptors, Glucocorticoid MeSH
- Saccharin MeSH
- Sweetening Agents MeSH
- Thiazines MeSH
Food constituents may cause a phenomenon of food-drug interactions. In the current study, we examined the effects of artificial sweeteners (aspartame, acesulfame, cyclamate, saccharin) on the aryl hydrocarbon receptor (AhR) and glucocorticoid receptor (GR)-dependent expression of CYP1A1 in human hepatocytes, hepatic HepG2 and intestinal LS174T cancer cell lines. Sweeteners were tested in concentrations up to those occurring in non-alcoholic beverages. Basal and ligand-inducible AhR- and GR-dependent reporter gene activation in stably transfected HepG2 and HeLa cells, respectively, were not affected by either of the sweeteners tested after 24h of incubation. The expression of CYP1A1 mRNA and protein in primary cultures of human hepatocytes and in LS174T and HepG2 cells was not induced by any of the tested sweeteners. Overall, aspartame, acesulfame, saccharin and cyclamate had no effects on CYP1A1 expression and transcriptional activities of AhR and GR. These data imply the safety of artificial sweeteners in terms of interference with AhR, GR and CYP1A1.
References provided by Crossref.org
