Effects of artificial sweeteners on the AhR- and GR-dependent CYP1A1 expression in primary human hepatocytes and human cancer cells
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24120730
DOI
10.1016/j.tiv.2013.10.001
PII: S0887-2333(13)00256-7
Knihovny.cz E-zdroje
- Klíčová slova
- 2,3,7,8-tetrachlorodibenzo-p-dioxin, AhR, Artificial sweeteners, Aryl hydrocarbon Receptor, Aryl hydrocarbon receptor, CAR, Constitutive androstane receptor, Cytochrome P450, ER, Food–drug interactions, GR, Glucocorticoid receptor, LXR, PXR, RXR, TCDD, estrogen receptor, glucocorticoid receptor, liver X receptor, pregnane X receptor, retinoic X receptor,
- MeSH
- aspartam farmakologie MeSH
- cyklamáty farmakologie MeSH
- cytochrom P-450 CYP1A1 genetika metabolismus MeSH
- hepatocyty účinky léků metabolismus MeSH
- interakce mezi potravou a léky * MeSH
- kultivované buňky MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- receptory aromatických uhlovodíků metabolismus MeSH
- receptory glukokortikoidů metabolismus MeSH
- sacharin farmakologie MeSH
- sladidla farmakologie MeSH
- thiaziny farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- acetosulfame MeSH Prohlížeč
- aspartam MeSH
- cyklamáty MeSH
- CYP1A1 protein, human MeSH Prohlížeč
- cytochrom P-450 CYP1A1 MeSH
- receptory aromatických uhlovodíků MeSH
- receptory glukokortikoidů MeSH
- sacharin MeSH
- sladidla MeSH
- thiaziny MeSH
Food constituents may cause a phenomenon of food-drug interactions. In the current study, we examined the effects of artificial sweeteners (aspartame, acesulfame, cyclamate, saccharin) on the aryl hydrocarbon receptor (AhR) and glucocorticoid receptor (GR)-dependent expression of CYP1A1 in human hepatocytes, hepatic HepG2 and intestinal LS174T cancer cell lines. Sweeteners were tested in concentrations up to those occurring in non-alcoholic beverages. Basal and ligand-inducible AhR- and GR-dependent reporter gene activation in stably transfected HepG2 and HeLa cells, respectively, were not affected by either of the sweeteners tested after 24h of incubation. The expression of CYP1A1 mRNA and protein in primary cultures of human hepatocytes and in LS174T and HepG2 cells was not induced by any of the tested sweeteners. Overall, aspartame, acesulfame, saccharin and cyclamate had no effects on CYP1A1 expression and transcriptional activities of AhR and GR. These data imply the safety of artificial sweeteners in terms of interference with AhR, GR and CYP1A1.
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