Homologous constitutive expression of Xyn III in Trichoderma reesei QM9414 and its characterization
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Enzyme Activation MeSH
- Endo-1,4-beta Xylanases genetics metabolism MeSH
- Gene Expression * MeSH
- Cloning, Molecular MeSH
- Hydrogen-Ion Concentration MeSH
- Enzyme Stability MeSH
- Temperature MeSH
- Trichoderma genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Endo-1,4-beta Xylanases MeSH
Xylanase III (Xyn III), a specific endoxylanase that belongs to family 10 of the glycoside hydrolases, was overexpressed in Trichoderma reesei QM9414 using a constitutive strong promoter of the gene encoding pyruvate decarboxylase (pdc). The maximum recombinant xylanase activity achieved was 817.2 ± 65.2 U/mL in the transformant fermentation liquid. The productivities of Xyn III accounted for approximately 53% of the total protein secreted by the recombinant. The enzyme was optimally active at 60 °C and pH 6. The recombinant Xyn III was stable at pH 5-8. This is the first report on the homologous expression of xyn3 in T. reesei QM9414. The properties of Xyn III make it promising in a variety of industrial use.
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Biotechnol Lett. 2013 Sep;35(9):1433-40 PubMed
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Bioresour Technol. 2013 Feb;130:161-7 PubMed
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Gene. 1989 Sep 1;81(1):83-95 PubMed
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