Xylanase III (Xyn III), a specific endoxylanase that belongs to family 10 of the glycoside hydrolases, was overexpressed in Trichoderma reesei QM9414 using a constitutive strong promoter of the gene encoding pyruvate decarboxylase (pdc). The maximum recombinant xylanase activity achieved was 817.2 ± 65.2 U/mL in the transformant fermentation liquid. The productivities of Xyn III accounted for approximately 53% of the total protein secreted by the recombinant. The enzyme was optimally active at 60 °C and pH 6. The recombinant Xyn III was stable at pH 5-8. This is the first report on the homologous expression of xyn3 in T. reesei QM9414. The properties of Xyn III make it promising in a variety of industrial use.

Shenzhen Key Laboratory of Microbial Genetic Engineering College of Life Sciences Shenzhen University Shenzhen Guangdong 518060 People's Republic of China

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Appl Microbiol Biotechnol. 2006 Oct;72(5):995-1003 PubMed

Gene. 1987;56(1):117-24 PubMed

J Microbiol Biotechnol. 2010 Oct;20(10):1415-23 PubMed

Mol Biol Rep. 2011 Nov;38(8):4991-7 PubMed

Anal Biochem. 1976 May 7;72:248-54 PubMed

Appl Microbiol Biotechnol. 2000 Sep;54(3):370-5 PubMed

Biochemistry (Mosc). 2012 Oct;77(10):1190-8 PubMed

Fungal Genet Biol. 2008 Jul;45(7):1094-102 PubMed

J Biosci Bioeng. 2011 Jun;111(6):654-7 PubMed

Eukaryot Cell. 2009 Dec;8(12):1837-44 PubMed

Protein Expr Purif. 2013 Dec;92(2):196-202 PubMed

Biotechnol Lett. 2013 Sep;35(9):1433-40 PubMed

Gene. 1987;61(2):155-64 PubMed

J Ind Microbiol Biotechnol. 2013 Jan;40(1):75-83 PubMed

Bioresour Technol. 2013 Feb;130:161-7 PubMed

J Biosci Bioeng. 2008 Apr;105(4):367-74 PubMed

J Microbiol Biotechnol. 2013 Feb;23(2):251-9 PubMed

Gene. 1989 Sep 1;81(1):83-95 PubMed

Appl Environ Microbiol. 2008 Nov;74(21):6554-62 PubMed

Microb Cell Fact. 2012 Jun 18;11:84 PubMed

Microbiol Rev. 1991 Jun;55(2):303-15 PubMed