Cell infiltration is a critical parameter for the successful development of 3D matrices for tissue engineering. Application of electrospun nanofibers in tissue engineering has recently attracted much attention. Notwithstanding several of their advantages, small pore size and small thickness of the electrospun layer limit their application for development of 3D scaffolds. Several methods for the pore size and/or electrospun layer thickness increase have been recently developed. Nevertheless, tissue engineering still needs emerging of either novel nanofiber-enriched composites or new techniques for 3D nanofiber fabrication. Forcespinning(®) seems to be a promising alternative. The potential of the Forcespinning(®) method is illustrated in preliminary experiment with mesenchymal stem cells.

Laboratory of Molecular Structure Characterization; Institute of Microbiology; Academy of Sciences of the Czech Republic; Prague Czech Republic

Laboratory of Tissue Engineering; Institute of Experimental Medicine; Academy of Science of the Czech Republic; Prague Czech Republic

Laboratory of Tissue Engineering; Institute of Experimental Medicine; Academy of Science of the Czech Republic; Prague Czech Republic; University Center for Energy Efficient Buildings ; The Czech Technical University Prague; Kladno Czech Republic

Laboratory of Tissue Engineering; Institute of Experimental Medicine; Academy of Science of the Czech Republic; Prague Czech Republic; University Center for Energy Efficient Buildings ; The Czech Technical University Prague; Kladno Czech Republic; Institute of Biophysics; 2nd Faculty of Medicine; Charles University Prague; Prague Czech Republic; Czech Technical University Prague; Faculty of Biomedical Engineering; Kladno Czech Republic

Technical University of Liberec; Faculty of Textile Engineering; Department of Nonwoven Textiles; Liberec Czech Republic; Technical University of Liberec; Institute for Nanomaterials; Advanced technologies and Innovation; Liberec Czech Republic

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