Bone regeneration is a long-term process requiring proper scaffolding and drug delivery systems. The current study delivers a three-dimensional (3D) scaffold prepared by blend centrifugal spinning loaded with the osteogenic supplements (OS) β-glycerol phosphate, ascorbate-2-phosphate and dexamethasone. The OS were successfully encapsulated into a fibrous scaffold and showed sustained release for 30 days. Furthermore, biological testing showed the osteoinductive properties of the scaffolds on a model of human mesenchymal stem cells and stimulatory effect on a model of osteoblasts. The osteoinductive properties were further proved in vivo in critical size defects of rabbits. The amount of bone trabecules was bigger compared to control fibers without OS. The results indicate that due to its long-term drug releasing properties, single step fabrication process and 3D structure, the system shows ideal properties for use as a cell-free bone implant in tissue-engineering.

Department of Cell Biology Faculty of Science Charles University Prague Czech Republic

Department of Histology and Embryology and Biomedical Center Faculty of Medicine in Pilsen Charles University Karlovarská 48 301 00 Pilsen Czech Republic

Institute of Biophysics 2nd Faculty of Medicine Charles University 5 Uvalu 84 150 06 Prague 5 Czech Republic

Laboratory of Tissue Engineering The Czech Academy of Sciences Institute of Experimental Medicine Videnska 1083 142 20 Prague 4 Czech Republic 420 2 9644 2387 420 2 9644 2387

University Center for Energy Efficient Buildings Czech Technical University Prague Trinecka 1024 273 43 Bustehrad Czech Republic

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