A cell-free nanofiber composite scaffold regenerated osteochondral defects in miniature pigs
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23499757
DOI
10.1016/j.ijpharm.2013.02.056
PII: S0378-5173(13)00209-3
Knihovny.cz E-zdroje
- MeSH
- buněčná diferenciace MeSH
- buňky kostní dřeně cytologie MeSH
- chondrocyty cytologie MeSH
- fibrin chemie MeSH
- fibroblastový růstový faktor 2 aplikace a dávkování MeSH
- inzulin aplikace a dávkování MeSH
- kolagen typu I chemie MeSH
- kyselina hyaluronová chemie MeSH
- liposomy MeSH
- mezenchymální kmenové buňky cytologie MeSH
- miniaturní prasata MeSH
- modul pružnosti MeSH
- nanovlákna aplikace a dávkování chemie MeSH
- polyvinylalkohol chemie MeSH
- prasata MeSH
- regenerace kostí * MeSH
- tkáňové podpůrné struktury MeSH
- viabilita buněk MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fibrin MeSH
- fibroblastový růstový faktor 2 MeSH
- inzulin MeSH
- kolagen typu I MeSH
- kyselina hyaluronová MeSH
- liposomy MeSH
- polyvinylalkohol MeSH
The aim of the study was to evaluate the effect of a cell-free hyaluronate/type I collagen/fibrin composite scaffold containing polyvinyl alcohol (PVA) nanofibers enriched with liposomes, basic fibroblast growth factor (bFGF) and insulin on the regeneration of osteochondral defects. A novel drug delivery system was developed on the basis of the intake effect of liposomes encapsulated in PVA nanofibers. Time-controlled release of insulin and bFGF improved MSC viability in vitro. Nanofibers functionalized with liposomes also improved the mechanical characteristics of the composite gel scaffold. In addition, time-controlled release of insulin and bFGF stimulated MSC recruitment from bone marrow in vivo. Cell-free composite scaffolds containing PVA nanofibers enriched with liposomes, bFGF, and insulin were implanted into seven osteochondral defects of miniature pigs. Control defects were left untreated. After 12 weeks, the composite scaffold had enhanced osteochondral regeneration towards hyaline cartilage and/or fibrocartilage compared with untreated defects that were filled predominantly with fibrous tissue. The cell-free composite scaffold containing PVA nanofibers, liposomes and growth factors enhanced migration of the cells into the defect, and their differentiation into chondrocytes; the scaffold was able to enhance the regeneration of osteochondral defects in minipigs.
Citace poskytuje Crossref.org
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