A cell-free nanofiber composite scaffold regenerated osteochondral defects in miniature pigs
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23499757
DOI
10.1016/j.ijpharm.2013.02.056
PII: S0378-5173(13)00209-3
Knihovny.cz E-resources
- MeSH
- Cell Differentiation MeSH
- Bone Marrow Cells cytology MeSH
- Chondrocytes cytology MeSH
- Fibrin chemistry MeSH
- Fibroblast Growth Factor 2 administration & dosage MeSH
- Insulin administration & dosage MeSH
- Collagen Type I chemistry MeSH
- Hyaluronic Acid chemistry MeSH
- Liposomes MeSH
- Mesenchymal Stem Cells cytology MeSH
- Swine, Miniature MeSH
- Elastic Modulus MeSH
- Nanofibers administration & dosage chemistry MeSH
- Polyvinyl Alcohol chemistry MeSH
- Swine MeSH
- Bone Regeneration * MeSH
- Tissue Scaffolds MeSH
- Cell Survival MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fibrin MeSH
- Fibroblast Growth Factor 2 MeSH
- Insulin MeSH
- Collagen Type I MeSH
- Hyaluronic Acid MeSH
- Liposomes MeSH
- Polyvinyl Alcohol MeSH
The aim of the study was to evaluate the effect of a cell-free hyaluronate/type I collagen/fibrin composite scaffold containing polyvinyl alcohol (PVA) nanofibers enriched with liposomes, basic fibroblast growth factor (bFGF) and insulin on the regeneration of osteochondral defects. A novel drug delivery system was developed on the basis of the intake effect of liposomes encapsulated in PVA nanofibers. Time-controlled release of insulin and bFGF improved MSC viability in vitro. Nanofibers functionalized with liposomes also improved the mechanical characteristics of the composite gel scaffold. In addition, time-controlled release of insulin and bFGF stimulated MSC recruitment from bone marrow in vivo. Cell-free composite scaffolds containing PVA nanofibers enriched with liposomes, bFGF, and insulin were implanted into seven osteochondral defects of miniature pigs. Control defects were left untreated. After 12 weeks, the composite scaffold had enhanced osteochondral regeneration towards hyaline cartilage and/or fibrocartilage compared with untreated defects that were filled predominantly with fibrous tissue. The cell-free composite scaffold containing PVA nanofibers, liposomes and growth factors enhanced migration of the cells into the defect, and their differentiation into chondrocytes; the scaffold was able to enhance the regeneration of osteochondral defects in minipigs.
References provided by Crossref.org
Electrospun Nanofibers Encapsulated with Natural Products: A Novel Strategy to Counteract Skin Aging
Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides
