Genotoxicity but not the AhR-mediated activity of PAHs is inhibited by other components of complex mixtures of ambient air pollutants
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
24472612
DOI
10.1016/j.toxlet.2014.01.028
PII: S0378-4274(14)00039-3
Knihovny.cz E-zdroje
- Klíčová slova
- Air pollution, Benzo[a]pyrene, Complex mixtures, DNA adducts, PAHs, PM2.5,
- MeSH
- aromatické hydroxylasy genetika metabolismus MeSH
- benzopyren chemie farmakokinetika toxicita MeSH
- cytochrom P-450 CYP1A1 genetika metabolismus MeSH
- cytochrom P450 CYP1B1 MeSH
- fluoreny chemie farmakokinetika toxicita MeSH
- komplexní směsi farmakokinetika toxicita MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- pevné částice chemie farmakokinetika toxicita MeSH
- plíce účinky léků enzymologie metabolismus fyziologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- receptory aromatických uhlovodíků genetika metabolismus MeSH
- regulace genové exprese enzymů účinky léků MeSH
- RNA chemie genetika MeSH
- testy genotoxicity metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- aromatické hydroxylasy MeSH
- benzo(k)fluoranthene MeSH Prohlížeč
- benzopyren MeSH
- CYP1A1 protein, human MeSH Prohlížeč
- CYP1B1 protein, human MeSH Prohlížeč
- cytochrom P-450 CYP1A1 MeSH
- cytochrom P450 CYP1B1 MeSH
- fluoreny MeSH
- komplexní směsi MeSH
- pevné částice MeSH
- receptory aromatických uhlovodíků MeSH
- RNA MeSH
In this study, we compared the genotoxicity and aryl hydrocarbon receptor (AhR)-dependent transcriptional changes of selected target genes in human lung epithelial A549 cells incubated for 24 h, either with extractable organic matter (EOMs) from airborne particles <2.5 μm (PM2.5) collected at four localities from heavily polluted areas of the Czech Republic or two representative toxic polycyclic aromatic hydrocarbons (PAHs) present in EOMs, benzo[a]pyrene (B[a]P) and benzo[k]fluoranthene (B[k]F). Genotoxic effects were determined using DNA adduct analysis or analysis of expression of selected AhR-related genes involved in bioactivation of PAHs (CYP1A1, CYP1B1) and transcriptional repression (TIPARP). Sampled localities differing in the extent and source of air pollution did not exhibit substantially different genotoxicity. DNA adduct levels induced by three subtoxic EOM concentrations were relatively low (1-5 adducts/10(8) nucleotides), compared to levels induced by similar concentrations of B[a]P, while B[k]F gave very low DNA adduct levels. Here, we compared genotoxicity and gene deregulation induced by complex mixtures containing PAHs with the effects of the comparable concentrations of individual PAHs. Our results suggested inhibition of formation of B[a]P-induced DNA adducts compared to individual B[a]P, probably attributable to competitive inhibition by other non-genotoxic EOM components. In contrast, induction of AhR target genes appeared not to be antagonized by the components of complex mixtures, as induction of CYP1A1, CYP1B1 and TIPARP transcripts reached maximum levels induced by PAHs.
Czech Technical University Prague Karlovo namesti 13 121 35 Prague 2 Czech Republic
Department of Chemistry Veterinary Research Institute Hudcova 296 70 621 00 Brno Czech Republic
Citace poskytuje Crossref.org
Novel gene sets improve set-level classification of prokaryotic gene expression data
