Phenotypic and genotypic characteristics of Arcanobacterium haemolyticum isolated from clinical samples in a Danish hospital
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- Arcanobacterium klasifikace genetika izolace a purifikace fyziologie MeSH
- DNA bakterií chemie genetika MeSH
- faktory virulence genetika MeSH
- fylogeneze MeSH
- infekce bakteriemi řádu Actinomycetales mikrobiologie MeSH
- intergenová DNA chemie genetika MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- nemocnice MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- RNA ribozomální 23S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- techniky typizace bakterií MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Dánsko MeSH
- Názvy látek
- DNA bakterií MeSH
- faktory virulence MeSH
- intergenová DNA MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
- RNA ribozomální 23S MeSH
Six Arcanobacterium haemolyticum strains isolated from six patients of two hospitals in Denmark were identified phenotypically, also including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and by genotypic methods. The latter were performed by sequencing 16S rDNA and glyceraldehyde 3-phosphate dehydrogenase encoding gene gap and by amplification of an A. haemolyticum specific region of 16S-23S rDNA intergenic spacer region and 23S rDNA. The six A. haemolyticum strains were further investigated for the presence of seven potential virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, collagen binding protein, neuraminidase A and neuraminidase H which appeared to be present in two (seven virulence genes), two (six virulence genes) and two strains (four virulence genes), respectively. The phenotypic and genotypic properties described in the present study might help to reliably identify and further characterize A. haemolyticum isolated from human patients, a species which seems to be of increasing importance.
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Eur J Clin Microbiol Infect Dis. 1998 Aug;17(8):578-82 PubMed
Ann Intern Med. 1986 Dec;105(6):867-72 PubMed
Folia Microbiol (Praha). 2010 Nov;55(6):666-8 PubMed
BMC Microbiol. 2011 Oct 26;11:239 PubMed
Int J Syst Evol Microbiol. 2011 Jun;61(Pt 6):1265-1274 PubMed
Int J Syst Evol Microbiol. 2013 Jun;63(Pt 6):2019-2024 PubMed
Berl Munch Tierarztl Wochenschr. 2014 Jan-Feb;127(1-2):56-60 PubMed
J Infect Dis. 1986 Dec;154(6):1037-40 PubMed
Diagn Microbiol Infect Dis. 2012 Jan;72(1):1-7 PubMed
J Clin Microbiol. 2009 Jan;47(1):124-8 PubMed
Antonie Van Leeuwenhoek. 2005 Aug;88(2):87-102 PubMed
Clin Microbiol Infect. 2010 Nov;16(11):1626-30 PubMed
Surg Infect (Larchmt). 2013 Jun;14(3):322-4 PubMed
Res Vet Sci. 2009 Oct;87(2):186-8 PubMed
Vet Microbiol. 2012 May 25;157(1-2):243-5 PubMed
J Gen Microbiol. 1982 Jun;128(6):1279-81 PubMed
Clin Infect Dis. 2009 Aug 15;49(4):543-51 PubMed
Mikrobiyol Bul. 2011 Jul;45(3):535-40 PubMed
Stand Genomic Sci. 2010 Sep 28;3(2):126-35 PubMed
J Infect. 2006 Aug;53(2):e69-74 PubMed
Ann Pharmacother. 2008 Nov;42(11):1697-702 PubMed
Int J Syst Evol Microbiol. 2012 Sep;62(Pt 9):2201-2205 PubMed
J Infect Dis. 1946 Jul-Aug;79:69-90 PubMed
BMC Microbiol. 2010 Oct 25;10:270 PubMed
