The newly described type strains Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, initially isolated from an anal swab of a harbor seal (Sammra et al. Int J Syst Evol Microbiol 65:4539-4543, 2015) and the genital tract of a rhinoceros (Sammra et al. Int J Syst Evol Microbiol 67:2093-2097, 2017), could be further characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and Fourier transform infrared (FT-IR) spectroscopy and by sequencing the genomic targets 16S-23S rDNA intergenic spacer region (ISR) and the genes rpoB, gap, and tuf. The two strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of both species remained unclear. However, the detection of specific spectra by MALDI-TOF MS and by FT-IR spectroscopy and the presented genotypic approaches might help to identify A. pinnipediorum and A. wilhelmae in the future and might elucidate the role these two species play in infections of animals.
- MeSH
- Arcanobacterium klasifikace genetika izolace a purifikace MeSH
- DNA bakterií MeSH
- fylogeneze MeSH
- mezerníky ribozomální DNA genetika MeSH
- ribozomální DNA genetika MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- Arcanobacterium izolace a purifikace MeSH
- bakteriologické techniky * MeSH
- Enterococcus izolace a purifikace MeSH
- Escherichia coli enzymologie izolace a purifikace účinky léků MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- Staphylococcus epidermidis izolace a purifikace účinky léků MeSH
- testování odbornosti laboratoří * metody statistika a číselné údaje MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- tabulky MeSH
In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region (ISR), the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis.
- MeSH
- Arcanobacterium genetika izolace a purifikace fyziologie MeSH
- bakteriální proteiny genetika MeSH
- diagnostické techniky molekulární metody MeSH
- DNA bakterií chemie genetika MeSH
- fylogeneze MeSH
- infekce bakteriemi řádu Actinomycetales mikrobiologie veterinární MeSH
- mastitida skotu mikrobiologie MeSH
- mezerníky ribozomální DNA chemie genetika MeSH
- mléko mikrobiologie MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- skot MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- techniky typizace bakterií metody MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Six Arcanobacterium haemolyticum strains isolated from six patients of two hospitals in Denmark were identified phenotypically, also including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and by genotypic methods. The latter were performed by sequencing 16S rDNA and glyceraldehyde 3-phosphate dehydrogenase encoding gene gap and by amplification of an A. haemolyticum specific region of 16S-23S rDNA intergenic spacer region and 23S rDNA. The six A. haemolyticum strains were further investigated for the presence of seven potential virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, collagen binding protein, neuraminidase A and neuraminidase H which appeared to be present in two (seven virulence genes), two (six virulence genes) and two strains (four virulence genes), respectively. The phenotypic and genotypic properties described in the present study might help to reliably identify and further characterize A. haemolyticum isolated from human patients, a species which seems to be of increasing importance.
- MeSH
- Arcanobacterium klasifikace genetika izolace a purifikace fyziologie MeSH
- DNA bakterií chemie genetika MeSH
- faktory virulence genetika MeSH
- fylogeneze MeSH
- infekce bakteriemi řádu Actinomycetales mikrobiologie MeSH
- intergenová DNA chemie genetika MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- nemocnice MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- RNA ribozomální 23S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- techniky typizace bakterií MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Dánsko MeSH
An Arcanobacterium haemolyticum strain isolated from a postcastrational lesion of a horse was identified phenotypically and genotypically. The latter was performed by sequencing the 16S-23S rDNA intergenic spacer region (ISR), by amplification of the gene encoding A. haemolyticum phospholipase D, by amplification of A. haemolyticum specific parts of ISR-23S rDNA and by amplification of the newly described CAMP factor family protein encoding gene of A. haemolyticum. This indicates (as described previously for seven additional A. haemolyticum strains; Hassan et al. 2009) that A. haemolyticum seems to occur also in infections of horses.
- MeSH
- Arcanobacterium fyziologie genetika izolace a purifikace klasifikace MeSH
- bakteriální proteiny genetika MeSH
- DNA bakterií genetika chemie MeSH
- fosfolipasa D genetika MeSH
- fylogeneze MeSH
- hemolýza MeSH
- hemolyziny genetika MeSH
- infekce bakteriemi řádu Actinomycetales mikrobiologie veterinární MeSH
- infekce chirurgické rány mikrobiologie veterinární MeSH
- kastrace škodlivé účinky veterinární MeSH
- koně mikrobiologie MeSH
- mezerníky ribozomální DNA genetika chemie MeSH
- molekulární sekvence - údaje MeSH
- nemoci koní mikrobiologie MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
