In the present study, a single Arcanobacterium (A.) pinnipediorum strain isolated from discharge of a jaw swelling of a grey seal pup (Halichoerus grypus) in England, UK, was identified. This strain was further characterized by phenotypical investigations, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), by Fourier transform infrared spectroscopy (FT-IR), and genotypically by sequencing the 16S rRNA gene and the genes gap encoding glyceraldehyde 3-phosphate dehydrogenase, tuf encoding elongation factor tu, and rpoB encoding the β subunit of bacterial RNA polymerase. The present study gives a first detailed characterization of the species A. pinnipediorum from a grey seal in the UK. However, the route of infection of the grey seal with the bacterial pathogen remains unclear.
- MeSH
- Arcanobacterium * genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Seals, Earless * genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- United Kingdom MeSH
Trueperella (T.) bernardiae is a well-known bacterial pathogen in infections of humans, rarely in animals. In the present study, five T. bernardiae isolates, isolated from five Peking ducks of four different farms, were identified by phenotypic properties, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and genotypically by sequencing the 16S ribosomal RNA (rRNA) gene, the superoxide dismutase A encoding gene sodA, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap. In addition, the T. bernardiae isolates could be identified with a newly developed loop-mediated isothermal amplification (LAMP) assay based on the gyrase encoding housekeeping gene gyrA. All these tests clearly identified the T. bernardiae isolates to the species level. However, the detection of the specific gene gyrA with the newly designed LAMP assay appeared with a high sensitivity and specificity, and could help to identify this bacterial species in human and animal infections in future. The importance of the T. bernardiae isolates for the clinical condition of the ducks and for the problems at farm level remains unclear.
- MeSH
- Actinomycetaceae MeSH
- Arcanobacterium * genetics MeSH
- Molecular Diagnostic Techniques MeSH
- Ducks * genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Nucleic Acid Amplification Techniques MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Beijing MeSH
The newly described type strains Arcanobacterium pinnipediorum DSM 28752T and Arcanobacterium wilhelmae DSM 102162T, initially isolated from an anal swab of a harbor seal (Sammra et al. Int J Syst Evol Microbiol 65:4539-4543, 2015) and the genital tract of a rhinoceros (Sammra et al. Int J Syst Evol Microbiol 67:2093-2097, 2017), could be further characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and Fourier transform infrared (FT-IR) spectroscopy and by sequencing the genomic targets 16S-23S rDNA intergenic spacer region (ISR) and the genes rpoB, gap, and tuf. The two strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of both species remained unclear. However, the detection of specific spectra by MALDI-TOF MS and by FT-IR spectroscopy and the presented genotypic approaches might help to identify A. pinnipediorum and A. wilhelmae in the future and might elucidate the role these two species play in infections of animals.
- MeSH
- Arcanobacterium classification genetics isolation & purification MeSH
- DNA, Bacterial MeSH
- Phylogeny MeSH
- DNA, Ribosomal Spacer genetics MeSH
- DNA, Ribosomal genetics MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Publication type
- Journal Article MeSH
- MeSH
- Arcanobacterium isolation & purification MeSH
- Bacteriological Techniques * MeSH
- Enterococcus isolation & purification MeSH
- Escherichia coli enzymology isolation & purification drug effects MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Staphylococcus epidermidis isolation & purification drug effects MeSH
- Laboratory Proficiency Testing * methods statistics & numerical data MeSH
- Check Tag
- Humans MeSH
- Publication type
- Tables MeSH
In the present study, three Arcanobacterium pluranimalium strains isolated from bovine milk samples of three cows of three farms (two cows with subclinical mastitis) could successfully be identified by phenotypical investigations, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis and genotypically by sequencing the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region (ISR), the β subunit of bacterial RNA polymerase encoding gene rpoB, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, the elongation factor tu encoding gene tuf, and the pluranimaliumlysin encoding gene pla. The latter could also be identified by a loop-mediated isothermal amplification (LAMP) assay. The presented phenotypic and genotypic approaches might support the identification of A. pluranimalium in future and might help to understand the role this species plays in bovine mastitis.
- MeSH
- Arcanobacterium genetics isolation & purification physiology MeSH
- Bacterial Proteins genetics MeSH
- Molecular Diagnostic Techniques methods MeSH
- DNA, Bacterial chemistry genetics MeSH
- Phylogeny MeSH
- Actinomycetales Infections microbiology veterinary MeSH
- Mastitis, Bovine microbiology MeSH
- DNA, Ribosomal Spacer chemistry genetics MeSH
- Milk microbiology MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Cattle MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Bacterial Typing Techniques methods MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Six Arcanobacterium haemolyticum strains isolated from six patients of two hospitals in Denmark were identified phenotypically, also including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and by genotypic methods. The latter were performed by sequencing 16S rDNA and glyceraldehyde 3-phosphate dehydrogenase encoding gene gap and by amplification of an A. haemolyticum specific region of 16S-23S rDNA intergenic spacer region and 23S rDNA. The six A. haemolyticum strains were further investigated for the presence of seven potential virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, collagen binding protein, neuraminidase A and neuraminidase H which appeared to be present in two (seven virulence genes), two (six virulence genes) and two strains (four virulence genes), respectively. The phenotypic and genotypic properties described in the present study might help to reliably identify and further characterize A. haemolyticum isolated from human patients, a species which seems to be of increasing importance.
- MeSH
- Arcanobacterium classification genetics isolation & purification physiology MeSH
- DNA, Bacterial chemistry genetics MeSH
- Virulence Factors genetics MeSH
- Phylogeny MeSH
- Actinomycetales Infections microbiology MeSH
- DNA, Intergenic chemistry genetics MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Hospitals MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- RNA, Ribosomal, 23S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
- Bacterial Typing Techniques MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Denmark MeSH
An Arcanobacterium haemolyticum strain isolated from a postcastrational lesion of a horse was identified phenotypically and genotypically. The latter was performed by sequencing the 16S-23S rDNA intergenic spacer region (ISR), by amplification of the gene encoding A. haemolyticum phospholipase D, by amplification of A. haemolyticum specific parts of ISR-23S rDNA and by amplification of the newly described CAMP factor family protein encoding gene of A. haemolyticum. This indicates (as described previously for seven additional A. haemolyticum strains; Hassan et al. 2009) that A. haemolyticum seems to occur also in infections of horses.
- MeSH
- Arcanobacterium physiology genetics isolation & purification classification MeSH
- Bacterial Proteins genetics MeSH
- DNA, Bacterial genetics chemistry MeSH
- Phospholipase D genetics MeSH
- Phylogeny MeSH
- Hemolysis MeSH
- Hemolysin Proteins genetics MeSH
- Actinomycetales Infections microbiology veterinary MeSH
- Surgical Wound Infection microbiology veterinary MeSH
- Castration adverse effects veterinary MeSH
- Horses microbiology MeSH
- DNA, Ribosomal Spacer genetics chemistry MeSH
- Molecular Sequence Data MeSH
- Horse Diseases microbiology MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
Tonzilofaryngitidy jsou jednou z nejčastějších indikací antibiotik v ambulantní praxi, avšak 50–60 % je indikováno u virových infekcí. Nadměrná a často neuvážená preskripce antibiotik je rizikem vzestupu rezistence baktérií zejména k makrolidům, což bylo pozorováno v České republice od konce devadesátých let. Intervenční programy zaměřené především na preskripci lékařů v ambulantních praxích již prokazují svůj význam, protože nárůst počtu rezistentních kmenů streptokoků a pneumokoků se od roku 2005 významně nezvyšuje. Indikace antibiotické léčby se zpřesnily rovněž možnostmi vyšetření C-reaktivního proteinu (CRP) a rychlých metod detekce streptokokového antigenu.
- MeSH
- Anti-Bacterial Agents administration & dosage therapeutic use MeSH
- Arcanobacterium immunology pathogenicity MeSH
- Bacterial Infections diagnosis etiology immunology MeSH
- Drug Resistance, Bacterial drug effects MeSH
- C-Reactive Protein analysis MeSH
- Corynebacterium diphtheriae immunology pathogenicity MeSH
- Diagnosis, Differential MeSH
- Child MeSH
- Drug Therapy methods MeSH
- Culture Techniques utilization MeSH
- Humans MeSH
- Primary Prevention methods MeSH
- Streptococcus pyogenes immunology pathogenicity MeSH
- Tonsillitis etiology drug therapy therapy MeSH
- Virus Diseases diagnosis etiology immunology MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- MeSH
- Aeromonas hydrophila isolation & purification classification MeSH
- Arcanobacterium isolation & purification MeSH
- Bacteriological Techniques MeSH
- Clostridium sordellii isolation & purification MeSH
- Laboratories standards MeSH
- Pseudomonas aeruginosa isolation & purification MeSH
- Quality Control MeSH
