BACKGROUND AND OBJECTIVE: Despite the fact that the molecular mechanism of low-level laser therapy (LLLT) is not yet known, the exploitation of phototherapy in clinical medicine and surgery is of great interest. The present study investigates the effects of LLLT on open skin wound healing in normal and diabetic rats. MATERIALS AND METHODS: Four round full-thickness skin wounds on dorsum were performed in male adult nondiabetic (n = 24) and diabetic (n = 24) Sprague-Dawley rats. AlGaInP (635 nm, wavelength; 5 J/cm(2), daily dose) was used to deliver power densities of 1, 5, and 15 mW/cm(2) three times daily until euthanasia. RESULTS: PMNL infiltration was lower in the irradiated groups (15 mW/cm(2)). The synthesis and organisation of collagen fibres were consecutively enhanced in the 5 mW/cm(2) and 15 mW/cm(2) groups compared to the others in nondiabetic rats. In the diabetic group the only significant difference was recorded in the ratio PMNL/Ma at 15 mW/cm(2). A significant difference in the number of newly formed capillaries in the irradiated group (5, 15 mW/cm(2)) was recorded on day six after injury compared to the control group. CONCLUSION: LLLT confers a protective effect against excessive inflammatory tissue response; it stimulates neovascularization and the early formation of collagen fibres.

1st Department of Internal Medicine Pavol Jozef Šafárik University Medical Faculty and Louis Pasteur University Hospital Košice Slovakia

1st Department of Surgery Pavol Jozef Šafárik University Medical Faculty and Louis Pasteur University Hospital Trieda SNP1 040 11 Košice Slovakia

Department of Cardiovascular Diseases International Clinical Research Center St Anne's University Hospital and Masaryk University Pekarska 53 656 91 Brno Czech Republic

Department of Forensic Medicine Pavol Jozef Šafárik University Medical Faculty Košice Slovakia

Department of Medical and Clinical Biophysics Pavol Jozef Šafárik University Medical Faculty Košice Slovakia

Department of Surgery Pavol Jozef Šafárik University Medical Faculty and 1st Private Hospital Košice Šaca a s Košice Slovakia

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Singer AJ, Clark RAF. Cutaneous wound healing. New England Journal of Medicine. 1999;341(10):738–746. PubMed

Greenhalgh DG. Wound healing and diabetes mellitus. Clinics in Plastic Surgery. 2003;30(1):37–45. PubMed

Kamal K, Powell RJ, Sumpio BE. The pathobiology of diabetes mellitus: implications for surgeons. Journal of the American College of Surgeons. 1996;183(3):271–289. PubMed

Meireles GCS, Santos JN, Chagas PO, Moura AP, Pinheiro ALB. Effectiveness of laser photobiomodulation at 660 or 780 nanometers on the repair of third-degree burns in diabetic rats. Photomedicine and Laser Surgery. 2008;26(1):47–54. PubMed

Akyol U, Güngörmüs M. The effect of low-level laser therapy on healing of skin incisions made using a diode laser in diabetic rats. Photomedicine and Laser Surgery. 2010;28:51–55. PubMed

Güngörmüs M, Akyol U. Effect of biostimulation on wound healing in diabetic rats. Photomedicine and Laser Surgery. 2009;27:607–610. PubMed

Alba-Loureiro TC, Martins EF, Landgraf RG, Jancar S, Curi R, Sannomiya P. Role of insulin on PGE2 generation during LPS-induced lung inflammation in rats. Life Sciences. 2006;78(6):578–585. PubMed

Cruz JWMC, Oliveira MA, Hohman TC, Fortes ZB. Influence of tolrestat on the defective leukocyte-endothelial interaction in experimental diabetes. European Journal of Pharmacology. 2000;391(1-2):163–174. PubMed

Toporcer T, Radoňak J. Vacuum asisted wound closure-overwiev of lesson and aplications. Casopis Lékaru Ceských. 2006;145:702–707. PubMed

Argenta LC, Morykwas MJ. Vacuum-assisted closure: a new method for wound control and treatment: clinical experience. Annals of Plastic Surgery. 1997;38(6):563–577. PubMed

Whelan HT, Smits RL, Jr., Buchman EV, et al. Effect of NASA light-emitting diode irradiation on wound healing. Journal of Clinical Laser Medicine and Surgery. 2001;19(6):305–314. PubMed

Peplow PV, Chung T-Y, Baxter GD. Laser photobiomodulation of wound healing: a review of experimental studies in mouse and rat animal models. Photomedicine and Laser Surgery. 2010;28(3):291–325. PubMed

Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M. Low-level laser therapy for wound healing: mechanism and efficacy. Dermatologic Surgery. 2005;31(3):334–340. PubMed

Gál P, Mokrý M, Vidinský B, et al. Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and corticosteroid-treated rats. Lasers in Medical Science. 2009;24:539–547. PubMed

Mester E, Spiry T, Szende B, Tota JG. Effect of laser rays on wound healing. The American Journal of Surgery. 1971;122(4):532–535. PubMed

Allendorf JD, Bessler M, Huang J, et al. Helium-neon laser irradiation at fluences of 1, 2, and 4 J/cm2 failed to accelerate wound healing as assessed by both wound contracture rate and tensile strength. Lasers in Surgery and Medicine. 1997;20:340–345. PubMed

Núñez SC, Nogueira GEC, Ribeiro MS, Garcez AS, Lage-Marques JL. He-Ne laser effects on blood microcirculation during wound healing: a method of in vivo study through laser Doppler flowmetry. Lasers in Surgery and Medicine. 2004;35(5):363–368. PubMed

Tuner J, Hode L. Low-Level Laser Therapy-Clinical Practice and Scientific Background. Spjutvägen, Sweden: Prima Books; 1999.

Karu TI, Afanasyeva NI, Kolyakov SF, Pyatibrat LV, Welser L. Changes in absorbance of monolayer of living cells induced by laser radiation at 633, 670, and 820 nm. IEEE Journal on Selected Topics in Quantum Electronics. 2001;7(6):982–988.

Karu TI, Pyatibrat LV, Kolyakov SF, Afanasyeva NI. Absorption measurements of cell monolayers relevant to mechanisms of laser phototherapy: reduction or oxidation of cytochrome c oxidase under laser radiation at 632.8 nm. Photomedicine and Laser Surgery. 2008;26(6):593–599. PubMed

Kreisler MB, Al Haj H, Noroozi N, Willershausen B, d’Hoedt B. Efficacy of low level laser therapy in reducing postoperative pain after endodontic surgery—a randomized double blind clinical study. International Journal of Oral and Maxillofacial Surgery. 2004;33(1):38–41. PubMed

Reddy GK, Stehno-Bittel L, Enwemeka CS. Laser photostimulation accelerates wound healing in diabetic rats. Wound Repair and Regeneration. 2001;9(3):248–255. PubMed

Kana JS, Hutschenreiter G, Haina D, Waidelich W. Effect of low-power density laser radiation on healing of open skin wounds in rats. Archives of Surgery. 1981;116(3):293–296. PubMed

Schindl A, Merwald H, Schindl L, Kaun C, Wojta J. Direct stimulatory effect of low-intensity 670 nm laser irradiation on human endothelial cell proliferation. British Journal of Dermatology. 2003;148(2):334–336. PubMed

Schlager A, Oehler K, Huebner K-U, Schmuth M, Spoetl L. Healing of burns after treatment with 670-nanometer low-power laser light. Plastic and Reconstructive Surgery. 2000;105(5):1635–1639. PubMed

Do Nascimento PM, Pinheiro ALB, Salgado MÂC, Ramalho LMP. A preliminary report on the effect of laser therapy on the healing of cutaneous surgical wounds as a consequence of an inversely proportional relationship between wavelength and intensity: histological study in rats. Photomedicine and Laser Surgery. 2004;22(6):513–518. PubMed

Lacjaková K, Bobrov N, Poláková M, et al. Effects of equal daily doses delivered by different power densities of low-level laser therapy at 670 nm on open skin wound healing in normal and corticosteroid-treated rats: a brief report. Lasers in Medical Science. 2010;25:761–766. PubMed

Vasilenko T, Slezák M, Kovác I, et al. The effect of equal daily dose achieved by different power densities of low-level laser therapy at 635 and 670 nm on wound tensile strength in rats: a short report. Photomedicine and Laser Surgery. 2010;28:281–283. PubMed

Lakyová L, Toporcer T, Tomečková V, Sabo J, Radoňak J. Low-level laser therapy for protection against skeletal muscle damage after ischemia-reperfusion injury in rat hindlimbs. Lasers in Surgery and Medicine. 2010;42:665–672. PubMed

Bisht D, Gupta SC, Misra V, Mital VP, Sharma P. Effect of low intensity laser radiation on healing of open skin wounds in rats. Indian Journal of Medical Research. 1994;100:43–46. PubMed

Hawkins DH, Abrahamse H. The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following Helium-Neon laser irradiation. Lasers in Surgery and Medicine. 2006;38(1):74–83. PubMed

Yasukawa A, Ohrui H, Koyama Y, Nagai M, Takakuda K. The effect of Low reactive-Level Laser Therapy (LLLT) with Helium-Neon laser on operative wound healing in a rat model. Journal of Veterinary Medical Science. 2007;69(8):799–806. PubMed

Gál P, Kilík R, Špaková T, et al. He-Ne laser irradiation accelerates inflammatory phase and epithelization of skin wound healing in rats. Biologia. 2005;60(6):691–696.

Yu W, Naim JO, Lanzafame RJ. Effects of photostimulation on wound healing in diabetic mice. Lasers in Surgery and Medicine. 1997;20:56–63. PubMed

Kilík R, Bober J, Gál P, et al. The influence of laser irradiation with different power densities on incisional wound healing in healthy and diabetic rats. Rozhledy v Chirurgii. 2007;86(7):384–387. PubMed

Pugliese LS, Medrado AP, Reis SRDA, Andrade ZDA. The influence of low-level laser therapy on biomodulation of collagen and elastic fibers. Pesquisa Odontologica Brasileira. 2003;17(4):307–313. PubMed

Maiya GA, Kumar P, Rao L. Effect of low intensity helium-neon (He–Ne) laser irradiation on diabetic wound healing dynamics. Photomedicine and Laser Surgery. 2005;23(2):187–190. PubMed

Marcos RL, Leal Junior ECP, De Moura Messias F, et al. Infrared (810 nm) low-level laser therapy in rat Achilles tendinitis: a consistent alternative to drugs. Photochemistry and Photobiology. 2011;87(6):1447–1452. PubMed

Demir H, Yaray S, Kirnap M, Yaray K. Comparison of the effects of laser and ultrasound treatments on experimental wound healing in rats. Journal of Rehabilitation Research and Development. 2004;41(5):721–727. PubMed

Stadler I, Lanzafame RJ, Evans R, et al. 830 nm irradiation increases the wound tensile strength in a diabetic murine model. Lasers in Surgery and Medicine. 2001;28(3):220–226. PubMed