Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2
Language English Country England, Great Britain Media electronic
Document type Journal Article
PubMed
24637612
DOI
10.1038/ncomms4477
PII: ncomms4477
Knihovny.cz E-resources
- MeSH
- ADP-Ribosylation Factor 6 MeSH
- ADP-Ribosylation Factors genetics metabolism MeSH
- Cell Line MeSH
- Endosomal Sorting Complexes Required for Transport genetics metabolism MeSH
- ErbB Receptors metabolism MeSH
- Exosomes enzymology genetics metabolism MeSH
- Phospholipase D genetics metabolism MeSH
- HIV Infections genetics metabolism virology MeSH
- HIV-1 physiology MeSH
- Humans MeSH
- Multivesicular Bodies enzymology genetics metabolism MeSH
- Cell Cycle Proteins genetics metabolism MeSH
- Calcium-Binding Proteins genetics metabolism MeSH
- Syntenins genetics metabolism MeSH
- Protein Transport MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- ADP-Ribosylation Factor 6 MeSH
- ADP-Ribosylation Factors MeSH
- ARF6 protein, human MeSH Browser
- Endosomal Sorting Complexes Required for Transport MeSH
- ErbB Receptors MeSH
- Phospholipase D MeSH
- PDCD6IP protein, human MeSH Browser
- phospholipase D2 MeSH Browser
- Cell Cycle Proteins MeSH
- Calcium-Binding Proteins MeSH
- Syntenins MeSH
Exosomes are small vesicles that are secreted by cells and act as mediators of cell to cell communication. Because of their potential therapeutic significance, important efforts are being made towards characterizing exosomal contents. However, little is known about the mechanisms that govern exosome biogenesis. We have recently shown that the exosomal protein syntenin supports exosome production. Here we identify the small GTPase ADP ribosylation factor 6 (ARF6) and its effector phospholipase D2 (PLD2) as regulators of syntenin exosomes. ARF6 and PLD2 affect exosomes by controlling the budding of intraluminal vesicles (ILVs) into multivesicular bodies (MVBs). ARF6 also controls epidermal growth factor receptor degradation, suggesting a role in degradative MVBs. Yet ARF6 does not affect HIV-1 budding, excluding general effects on Endosomal Sorting Complexes Required for Transport. Our study highlights a novel pathway controlling ILV budding and exosome biogenesis and identifies an unexpected role for ARF6 in late endosomal trafficking.
References provided by Crossref.org
Inclusion Biogenesis, Methods of Isolation and Clinical Application of Human Cellular Exosomes
