Sulfur mustard (SM) is a blister agent with cytotoxic mechanism of action. There is no suitable treatment based on administration of an antidote. In this study, Wistar rats were exposed to SM in doses of 0-40 mg/kg body weight and treated with the compound HI-6. The treatment provided no significant effect on ferric reducing antioxidant power of blood and plasma. However, HI-6 caused an increase in the level of thiobarbituric acid reactive substances. This stressogenic response was presumably the cause of the significant elevation of the blood level of both glutathione reductase and reduced glutathione. HI-6 appears to be suitable for enhancing prophylactically oxidative stress protection from small oxidative insult.

Faculty of Military Health Sciences University of Defense Trebesska 1575 50001 Hradec Kralove Czech Republic

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Bhattacharya R, Rao PV, Pant SC, Kumar P, Tulsawani RK, Pathak U, Kulkami A, Vijayaraghavan R. Protective effects of amifostine and its analogues on sulfur mustard toxicity in vitro and in vivo. Toxicol Appl Pharmacol. 2001;176:24–33. PubMed

Bogavac M, Lakic N, Simin N, Nikoilc A, Sudji J, Boin B. Biomarkers of oxidative stress in amniotic fluid and complications in pregnancy. J Matern Fetal Neonatal Med. 2012;25(1):104–108. PubMed

Catala A. Lipid peroxidation of membrane phospholipids in the vertebrate retina. Front Biosci. 2011;3:52–60. PubMed

Chen HC, Bai DY, Jiang YP. Effects of HI-6 on muscle acetylcholine receptor: analysis on minimal reaction model. Zhongguo Yao Li Xue Bao. 1996;17:428–431. PubMed

Collins A, Oscoz1 AA, Brunborg G, Gaiva I, Giovannelli L, Kruszewski M, Smith C, Stetina R. The comet assay: topical issues. Mutagenesis. 2008;23:143–151. PubMed

Gautam A, Gupta A, Lomash V, Pant SC, Vijayaraghavan R. Prophylactic efficacy of combination of DRDE-07 and its analogues with amifostine against sulphur mustard induced systemic toxicity. Indian J Exp Biol. 2010;48:752–761. PubMed

Gautam A, Vijayaraghavan R. Prophylactic effect of gossypin against percutaneously administered sulfur mustard. Biomed Environ Sci. 2007;20:250–259. PubMed

Korkamaz A, Kunak ZI, Paredes SD, Yaren H, Tan DX, Reiter RJ. The use of melatonin to combat mustard toxicity. Neuroendocrinol Lett. 2008b;29:614–619. PubMed

Korkmaz A, Yaren H, Kunak Z, Uysal B, Kurt B, Topal T, Kenar L, Ucar E, Oter S. Epigenetic perturbations in the pathogenesis of mustard toxicity; hypothesis and preliminary results. Interdiscip Toxicol. 2008a;1:236–241. PubMed PMC

Oke SL, Tracey KJ. The inflammatory reflex and the role of complementary and alterantive medical therapies. Ann NY Acad Sci. 2009;1172:172–180. PubMed PMC

Paromov V, Suntres Z, Smith M, Stone WL. Sulfur mustard toxicity following dermal exposure: role of oxidative stress, and antioxidant therapy. J Burns Wounds. 2007;7:e7. PubMed PMC

Pohanka M. Cholinesterases, a target of pharmacology and toxicology. Biomed Pap Olomouc. 2011;155:219–230. PubMed

Pohanka M. Alpha7 nicotinic acetylcholne receptor is a target in pharmacology and toxicology. Int J Mol Sci. 2012;13:2219–2238. PubMed PMC

Pohanka M, Sobotka J, Stetina R. Sulfur mustard induced oxidative stress and its alteration by epigallocatechin gallate. Toxicol Lett. 2011a;201:105–109. PubMed

Pohanka M, Sobotka J, Svobodova H, Stetina R. Investigation of oxidative stress in blood, brain, kidney, and liver after oxime antidote HI-6 application in a mouse experimental model. Drug Chem Toxicol. 2011b;34:255–260. PubMed

Pohanka M, Sobotka J, Jilkova M, Stetina R. Oxidative stress after sulfur mustard intoxication and its reduction by melatonin: efficacy of antioxidant therapy during serious intoxication. Drug Chem Toxicol. 2011c;34:85–91. PubMed

Shohrati M, Ghanei M, Shamspour N, Babaei F, Abadi MN, Jafari M, Harandi AA. Glutathione and malondialdehyde levels in late pulmonary complications of sulfur mustard intoxication. Lung. 2009;188:77–83. PubMed

Shohrati M, Ganei M, Shamspour N, Jafari M. Activity and function in lung injuries due to sulphur mustard. Biomarkers. 2008;13:728–733. PubMed

Tewari-Singh N, Agarwal C, Huang J, Day BJ, White CW, Agarwal R. Efficacy of glutathione in ameliorating sulfur mustard analog-induced toxicity in cultured skin epidermal cells and in SKH-1 mouse skin in vivo. J Pharmacol Exp Ther. 2011;336:450–459. PubMed PMC

Wagner S, Kufleitner J, Zensi A, Dadparvar M, Wien S, Bungert J, Vogel T, Worek F, Kreiter J, von Briesen H. Nanoparticulate transport of oximes over an in vitro blood-brain barrier model. PLoS One. 2010;5:e14213. PubMed PMC