The role of Ca(2+) and Na (+) membrane transport in brook trout (Salvelinus fontinalis) spermatozoa motility
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Amiloride MeSH
- Analysis of Variance MeSH
- Biological Transport physiology MeSH
- Sodium Chloride metabolism pharmacology MeSH
- Egtazic Acid MeSH
- Sperm Motility drug effects physiology MeSH
- Statistics, Nonparametric MeSH
- Trout physiology MeSH
- Sucrose metabolism pharmacology MeSH
- Spermatozoa drug effects physiology MeSH
- Calcium metabolism pharmacology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amiloride MeSH
- Sodium Chloride MeSH
- Egtazic Acid MeSH
- Sucrose MeSH
- Calcium MeSH
The role of environmental ion composition and osmolality in Ca(2+) signaled activation was assessed in spermatozoa of brook trout Salvelinus fontinalis. Milt from ten mature males was obtained by abdominal massage. Spermatozoa motility was evaluated in 0, 100, and 300 mOsm/kg NaCl or sucrose solutions, buffered by 10 mM Tris-HCl pH 8.5. For investigation of spermatozoa reaction to external Ca(2+) concentration, 2 mM ethylene glycol tetraacetic acid (EGTA) was added to the activation media as a calcium ions chelator. For investigation of the effect of external Na(+) concentration in conditions of low external Ca(2+), 100 µM amiloride was added to the EGTA-containing solutions as a Na(+) transport blocker. Low motility was observed in sucrose (Na(+) free) solutions containing 2 mM EGTA but not in Na(+) solutions containing 2 mM EGTA. Addition of amiloride led to significantly increased motility (P < 0.05) compared with sucrose (Na(+) free) solutions containing 2 mM EGTA. We conclude that Na(+) transport in Ca(2+)-free solutions plays a regulatory role in brook trout spermatozoa activation. The influence of competitive Na(+) and Ca(2+) transport on the control of spermatozoa activation requires further study with respect to its application for improvement of artificial activation and storage media.
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