OBJECTIVES: The aim of this study was to evaluate the relationships of the T-1131C (rs662799) polymorphism variants of apolipoprotein A5 (Apo A5) gene and variants of apolipoprotein E (Apo E) gene common polymorphism (rs429358, rs7412) to selected hemostatic markers. STUDY DESIGN AND METHODS: We examined 590 asymptomatic dyslipidemic patients, subsequently divided into MetS+ (n=146) and MetS- (n=444) groups according to the criteria for identification of the metabolic syndrome (MetS). We compared variant frequencies and differences in levels of hemostatic markers according to Apo A5, Apo E and Apo A5/Apo E common variants. RESULTS: The -1131C Apo A5 minor variant was associated with elevated tissue plasminogen activator (tPA) in comparison to TT genotype (p<0.001), but not in the MetS+ group. The analysis of Apo A5/Apo E common variants in all subjects revealed that the presence of -1131C minor allele has always been associated with higher levels of tPA in comparison with T allele, regardless of Apo E genotype. Also the presence of minor Apo E2 allele led to elevated tPA concentrations in both T and C carriers. In addition, common -1131C/E2 variant was associated with the highest tPA levels. CONCLUSION: We demonstrated a remarkable association especially between the -1131C Apo A5 variant and increased tPA levels in asymptomatic dyslipidemic patients.

3rd Department of Internal Medicine Faculty of Medicine and Dentistry Palacky University Olomouc and University Hospital Olomouc 1 P Pavlova 6 775 20 Olomouc Czech Republic

Department of Clinical Biochemistry University Hospital Olomouc 1 P Pavlova 6 775 20 Olomouc Czech Republic

Department of Haemato Oncology Faculty of Medicine and Dentistry Palacky University Olomouc and University Hospital Olomouc 1 P Pavlova 6 775 20 Olomouc Czech Republic

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Apolipoprotein A5 fifteen years anniversary: Lessons from genetic epidemiology