The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats
Language English Country Canada Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- antioxidants, antioxydants, apoptose, apoptosis, catechin, catéchine, culture cellulaire primaire, epigallocatechin gallate, hepatectomy, hepatocytes, hépatectomie, hépatocytes, le gallate d’épigallocatéchine, liver regeneration, oxidative stress, primary cell culture, régénération du foie, stress oxydatif,
- MeSH
- Antioxidants administration & dosage toxicity MeSH
- DNA biosynthesis MeSH
- Hepatectomy * MeSH
- Hepatocytes drug effects enzymology metabolism MeSH
- Caspase 3 metabolism MeSH
- Caspase 7 metabolism MeSH
- Catechin administration & dosage analogs & derivatives toxicity MeSH
- Rats MeSH
- Oxidative Stress drug effects MeSH
- Rats, Wistar MeSH
- Primary Cell Culture MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antioxidants MeSH
- DNA MeSH
- epigallocatechin gallate MeSH Browser
- Caspase 3 MeSH
- Caspase 7 MeSH
- Catechin MeSH
Epigallocatechin gallate (EGCG) is an antioxidant found in green tea. In this study, male Wistar rats were subjected either to partial hepatectomy (PHx), or a sham operation (LAP). Twenty-four hours after surgery, hepatocytes were isolated and treated with various concentrations of EGCG for up to 72 h. We then measured markers of cell viability, oxidative stress, DNA synthesis, and caspase activity. Morphological criteria, cell viability tests, and albumin synthesis revealed toxicity starting at 10 μmol/L. DNA synthesis was higher in hepatocytes isolated from rats after PHx and inhibited by EGCG. Furthermore, EGCG increased the activity of caspases 3 and 7, seen more in hepatocytes from PHx rats. In conclusion, EGCG at a concentration of 10 μmol/L was toxic for hepatocytes isolated from both PHx and LAP rats.
References provided by Crossref.org
In vitro toxicity of epigallocatechin gallate in rat liver mitochondria and hepatocytes
