The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats
Jazyk angličtina Země Kanada Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24853265
DOI
10.1139/cjpp-2014-0069
Knihovny.cz E-zdroje
- Klíčová slova
- antioxidants, antioxydants, apoptose, apoptosis, catechin, catéchine, culture cellulaire primaire, epigallocatechin gallate, hepatectomy, hepatocytes, hépatectomie, hépatocytes, le gallate d’épigallocatéchine, liver regeneration, oxidative stress, primary cell culture, régénération du foie, stress oxydatif,
- MeSH
- antioxidancia aplikace a dávkování toxicita MeSH
- DNA biosyntéza MeSH
- hepatektomie * MeSH
- hepatocyty účinky léků enzymologie metabolismus MeSH
- kaspasa 3 metabolismus MeSH
- kaspasa 7 metabolismus MeSH
- katechin aplikace a dávkování analogy a deriváty toxicita MeSH
- krysa rodu Rattus MeSH
- oxidační stres účinky léků MeSH
- potkani Wistar MeSH
- primární buněčná kultura MeSH
- viabilita buněk účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- DNA MeSH
- epigallocatechin gallate MeSH Prohlížeč
- kaspasa 3 MeSH
- kaspasa 7 MeSH
- katechin MeSH
Epigallocatechin gallate (EGCG) is an antioxidant found in green tea. In this study, male Wistar rats were subjected either to partial hepatectomy (PHx), or a sham operation (LAP). Twenty-four hours after surgery, hepatocytes were isolated and treated with various concentrations of EGCG for up to 72 h. We then measured markers of cell viability, oxidative stress, DNA synthesis, and caspase activity. Morphological criteria, cell viability tests, and albumin synthesis revealed toxicity starting at 10 μmol/L. DNA synthesis was higher in hepatocytes isolated from rats after PHx and inhibited by EGCG. Furthermore, EGCG increased the activity of caspases 3 and 7, seen more in hepatocytes from PHx rats. In conclusion, EGCG at a concentration of 10 μmol/L was toxic for hepatocytes isolated from both PHx and LAP rats.
Citace poskytuje Crossref.org
In vitro toxicity of epigallocatechin gallate in rat liver mitochondria and hepatocytes
